Skip to main content
SpringerLink
Log in

Assessment of gastric bleeding in rats: Effects of cyclooxygenase inhibitors and 16,16-dimethyl prostaglandin E2 on gastric bleeding

  • Ulcerogenicity
  • Published:
Agents and Actions Aims and scope Submit manuscript

Abstract

Gastric bleeding caused by cyclooxygenase inhibitors has been assessed by a novel method. Rats are adapted to a strict light-dark cycle with limited access to food to reduce thestress associated with starvation. Such animals are then labeled with51Cr-red blood cells from donor animals and dosed with the compound under evaluation. After 24 hr. animals are sacrificed and the amount of blood that has accumulated in the lumen of the cecum is quantitated. The potency of cyclooxygenase inhibitors in this assay to cause gastric bleeding is as follows: indomethacin>piroxicam>naproxen>ibuprofen>diflunisal which is similar to their antiinflammatory potency in the rat. In addition, the protective activity of PGE2 on indomethacin-induced gastric bleeding is clearly shown by this method.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. C. A. Winter, E. A. Rigley and G. W. Nuss,Antiinflammatory and antipyretic activities of indomethacin, 1-(p-chlorobenzoyl)-5-methoxy-2-methyl-indole-3-acetric acid. J. Pharmacol. Expt. Ther.141, 369–376 (1963).

    Google Scholar 

  2. J. Edelson and J. F. Douglas,Measurement of gatrointestinal blood loss in the rat: The effect of aspirin, phenylbutazone and seclazone. J. Pharm. Exp. Therap.184, 449–452 (1973).

    Google Scholar 

  3. R. D. Smyth, P. P. Mathew, R. L. Procacci, G. S. Carr and N. H. Reavey-Cantwell,Comparative effects of fenclorac and indomethacin on gastrointestinal blood loss in the rat. Toxicology and Appl. Pharmacol.38, 507–515 (1976).

    Article  Google Scholar 

  4. B. M. Phillips,Aspirin-induced gastrointestinal microbleeding in dogs. Toxicology and Applied Pharmacol.24, 182–189 (1973).

    Article  Google Scholar 

  5. B. M. Phillips and B. T. Palermo,Physical form as a determinant of effect of buffered acetylsalicylate formulations on G.I. microbleeding. J. of Pharmaceutical Sciences66, 124–166 (1977).

    Google Scholar 

  6. B. J. R. Whittle,Relationship between the prevention of rat gastric erosions and the inhibition of acid secretion by prostaglandins. E. J. Pharmacol.40, 233–239 (1976).

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Immunology and Inflammation Research, Merck Institute for Therapeutic Research, P.O. Box 2000, 07065, Rahway, New Jersey, USA

    R. J. Bonney, S. Glinka, S. Hopple, P. Bailey, J. L. Humes & L. W. Argenbright

Authors
  1. R. J. Bonney
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. Glinka
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. Hopple
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. P. Bailey
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. J. L. Humes
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. L. W. Argenbright
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bonney, R.J., Glinka, S., Hopple, S. et al. Assessment of gastric bleeding in rats: Effects of cyclooxygenase inhibitors and 16,16-dimethyl prostaglandin E2 on gastric bleeding. Agents and Actions 21, 310–313 (1987). https://doi.org/10.1007/BF01966500

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01966500

Keywords

Search

Navigation