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Theα isoform of protein kinase C inhibits histamine-stimulated adenylate cyclase activity in a particulate fraction of the human gastric cancer cell line HGT-1

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Abstract

The isoform of protein kinase C responsible for the inhibition of histamine-stimulated adenylate cyclase by the phorbol ester, 12-O-tetradecanoylphorbol 13-acetate (TPA), has been investigated in a particulate fraction prepared from the human gastric cancer cell line HGT-1. Theα and∈ isoforms of protein kinase C were detected in HGT-1 cells and in a 40,000 × g particulate fraction by immunoblotting procedures. The inhibitory effect of TPA on histamine-stimulated adenylate cyclase was enhanced by the presence of Ca2+, but decreased in a concentrationdependent manner by anti-peptide antibody to protein kinase Cα, but not to protein kinase C∈. Addition of Ca2+ and TPA to the 40,000 × g particulate fraction stimulated the phosphorylation of the protein kinase C substrate myelin basic peptide 4–14. Protein kinase Cα is probably the isoform responsible for inhibition of histamine-stimulated adenylate cyclase in HGT-1 cells.

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  1. J. M. Williams

    Present address: Renal Research Laboratories, Clinical Research Block, Queen Elizabeth Hospital, B15 2TH, Birmingham, UK

Authors and Affiliations

  1. Pharmaceutical Sciences Institute, Aston University, B4 7ET, Birmingham, UK

    J. P. McKenna, J. M. Williams & P. J. Hanson

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  1. J. P. McKenna
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  2. J. M. Williams
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  3. P. J. Hanson
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McKenna, J.P., Williams, J.M. & Hanson, P.J. Theα isoform of protein kinase C inhibits histamine-stimulated adenylate cyclase activity in a particulate fraction of the human gastric cancer cell line HGT-1. Inflamm Res 44, 66–69 (1995). https://doi.org/10.1007/BF01793214

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  • DOI: https://doi.org/10.1007/BF01793214

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