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A rapid and simple method for the isolation of pure eosinophilic leukocytes from horse blood

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Summary

An improved and short method is described for the isolation of intact eosinophilic leukocytes from horse blood with high yield (1–1.5 g/20 1). Viability and purity of the preparations were verified by light and electron microscopy and by the trypan blue exclusion test. Isolated eosinophils were 98–100% pure, intact and viable, and they could be shown to phagocytise immune-complexes.

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Authors and Affiliations

  1. Physiologisch-chemisches Institut, Universität Freiburg, CH-1700, Freiburg, Switzerland

    A. Jörg, P. Portmann, G. Fellay, J. L. Dreyer & J. Meyer

Authors
  1. A. Jörg
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  2. P. Portmann
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  3. G. Fellay
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  4. J. L. Dreyer
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  5. J. Meyer
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Additional information

The authors are indebted to the ‘Schweizerischer Nationalfonds zur Förderung der wissenschaftlichen Forschung’ for financial support.

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Jörg, A., Portmann, P., Fellay, G. et al. A rapid and simple method for the isolation of pure eosinophilic leukocytes from horse blood. Experientia 34, 1654–1656 (1978). https://doi.org/10.1007/BF02034734

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  • DOI: https://doi.org/10.1007/BF02034734

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