Summary
Lysine modification by increasing quantities of succinic anhydride in theEscherichia coli ribosomal protein L7/L12 produces loss of its ability in reconstitution of elongation-factor-G-dependent GTP hydrolysis and polyphenylalanine synthesis activities, showing lower antigenicity and loss of antigenic determinants.
Similar content being viewed by others
References
E. Hamel, M. Koka and T. Nakamoto, J. biol. Chem.247, 805 (1972).
H. Weissbach, B. Redfield, E. Yamasaki, R.C. Davis, Jr, S. Petska and N. Brot, Archs Biochem. Biophys.149, 110 (1972).
C. Bernabeu, D. Vázquez and J.P.G. Ballesta, Eur. J. Biochem.69, 233 (1976).
A.R. Subramanian, J. molec. Biol.95, 1 (1975).
L. Ramanathan, R.B. Guyer, E.G. Buss and C.O. Clagett, Molec. Immun.17, 267 (1980).
I.M. Hunneyball and D.R. Stanworth, Immunology30, 881 (1976).
M.H. Klapper and I.M. Klotz, Meth. Enzym.25B, 531 (1972).
J. Modolell and D. Vázquez, J. biol. Chem.248, 488 (1973).
Abbreviations used: HEPES, N-2-hydroxyethylpiperazine-N′-2-ethanesulphonic acid; DTT, dithiothreitol; EDTA, ethylenediaminetetra-acetic acid, disodium salt.
J.A. Pintor-Toro, D. Vázquez and E. Palacian, FEBS Lett.87, 125 (1978).
J.P.G. Ballesta, V. Montejo, F. Hernández and D. Vázquez, Eur. J. Biochem.42, 167 (1974).
F. Sánchez-madrid, R. Reyes, P. Conde and J.P.G. Ballesta, Eur. J. Biochem.98, 409 (1979).
S. Hjertén and R. Mosbach, Analyt. Biochem.3, 109 (1962).
B.J. Davis, Ann. N.Y. Acad. Sci.121, 404 (1964).
A.H. Reisner, P. Nemel and C. Bucholtz, Analyt. Biochem.64, 509 (1975).
C.A. Luer and K. Wong, Biochemistry18, 2019 (1979).
Author information
Authors and Affiliations
Additional information
This study was supported in part by ‘Fondo de investigaciones sanitarias del INSALUD’.
Rights and permissions
About this article
Cite this article
Pintor-Toro, J.A., Sanchez-Madrid, F., Vidal, M.A. et al. Studies on the modification ofEscherichia coli ribosomal protein L7/L12 by succinic anhydride. Experientia 38, 241–243 (1982). https://doi.org/10.1007/BF01945089
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF01945089