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Survival of rabbit embryos after rapid freezing and thawing

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Summary

Frozen storage of rabbit embryos at the 16-cell stage in 2.0 M dimethylsulfoxide (DMSO) in phosphate-buffered saline (PBS) was achieved by a 2-step procedure. After storage for 10 days at−196°C they were revived by rapidly thawing at 500°C/min. On transfer of these embryos to pseudopregnant foster mothers, 50% survived to term. The difference in in vivo survival between frozen-thawed and frozen-thawed-cultured embryos was not significant.

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Literatur

  1. Whittingham, D.G., Leibo, S.P., and Mazur, P., Science178 (1972) 411.

    Article  CAS  PubMed  Google Scholar 

  2. Whittingham, D.G., J. Reprod. Fert.43 (1975) 575.

    Article  CAS  Google Scholar 

  3. Bank, H., and Maurer, R.R., Expl Cell Res.89 (1974) 188.

    Article  CAS  Google Scholar 

  4. Maurer, R.R., and Haseman, J.K., Biol. Reprod.14 (1976) 256.

    Article  CAS  PubMed  Google Scholar 

  5. Whittingham, D.G., and Adams, C.E., J. Reprod. Fert.47 (1976) 269.

    Article  CAS  Google Scholar 

  6. Tsunoda, Y., and Sugie, T., J. Reprod. Fert.49 (1977) 173.

    Article  CAS  Google Scholar 

  7. Bilton, R.J., and Moore, N.W., J. Reprod. Fert.46 (1976) 537.

    Article  CAS  Google Scholar 

  8. Willadsen, S.M., Polge, C., and Rowson, L.E.A., in: Control of reproduction in the cow, p. 427. Ed. J.M. Streenan. Martinus Nijhoff, The Hague/Boston/London 1978.

    Chapter  Google Scholar 

  9. Willadsen, S.M., Polge, C., Rowson, L.E.A., and Moor, R.M., J. Reprod. Fert.46 (1976) 151.

    Article  CAS  Google Scholar 

  10. Bilton, R.J., and Moore, N.W., Aust. J. biol. Sci.29 (1977) 125.

    Article  Google Scholar 

  11. Wood, M.J., and Farrant, J., Cryobiology17 (1980) 178.

    Article  CAS  PubMed  Google Scholar 

  12. Kasai, M., Niwa, K., and Iritani, A., J. Reprod. Fert.59 (1980) 51.

    Article  CAS  Google Scholar 

  13. Kennelly, J.J., and Foote, R.H., J. Reprod. Fert.9 (1965) 177.

    Article  CAS  Google Scholar 

  14. Agrawal, K.P., Mongha, I.V., and Bhattacharyya, N.K., Indian J. exp. Biol.17 (1979) 1314.

    CAS  PubMed  Google Scholar 

  15. Whittingham, D.G., Nature, Lond.233 (1971) 125.

    Article  CAS  PubMed  Google Scholar 

  16. Rao, V.H., Agrawal, K.P., Mongha, I.V., and Bhattacharyya, N.K., Curr. Sci.54 (1982) 193.

    Google Scholar 

  17. Whittingham, D.G., in: Freezing of mammalian embryos, p. 97. Ciba Foundation Symposium No. 52 (new series). Elsevier, Amsterdam 1977.

    Google Scholar 

  18. Leibo, S.P., and Mazur, P., in: Methods in Mammalian Reproduction, p. 179. Ed. J.C. Daniel, Jr. Academic Press, New York 1978.

    Chapter  Google Scholar 

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Authors and Affiliations

  1. Indian Veterinary Research Institute, 243 122, Izatnagar, India

    V. H. Rao, I. V. Mongha, M. R. Ansari & N. K. Bhattacharyya

Authors
  1. V. H. Rao
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  2. I. V. Mongha
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  3. M. R. Ansari
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  4. N. K. Bhattacharyya
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Additional information

Acknowledgment. The authors thank the Director for the facilities. VHR is supported by Council of Scientific and Industrial Research (India).

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Rao, V.H., Mongha, I.V., Ansari, M.R. et al. Survival of rabbit embryos after rapid freezing and thawing. Experientia 40, 821–822 (1984). https://doi.org/10.1007/BF01951970

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  • DOI: https://doi.org/10.1007/BF01951970

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