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Serum-induced stimulation of snRNA synthesis in mouse 3T3 fibroblasts

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Summary

Small nuclear RNAs (snRNAs) from quiescent and serum-stimulated 3T3 cultures, labeled with [3H]uridine ([3H]U), were electrophoresed in polyacrylamide-urea slab gels and revealed by staining with ethidium bromide and by fluorography, Judged by labeling with [3H]U, synthesis of 7S and U1-U6 RNAs was very low or absent in quiescent cultures. The serum-induced transition of 3T3 cells from a resting to a growing state was accompanied by an early, apparently sequential stimulation of snRNA synthesis; stimulated synthesis of 7S, U1, U2, U3, U4 and U6 RNAs coincided in time with serum-induced stimulation of 45S pre-ribosomal RNA (pre-rRNA) and heterogeneous nuclear RNA (hnRNA) synthesis.

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Literatur

  1. Acknowledgments. We are grateful to Dr Monique Jacob for the gift of the snRNA preparation from HeLa cells and to M.O. Jenni for drawing the figures. This work was supported by grant 3.072-0.81 of the Swiss National Science Foundation.

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  1. J. -M. Tiercy

    Present address: Department of Neurobiology, Stanford University School of Medicine, 94305, Stanford, California, USA

Authors and Affiliations

  1. Department of Molecular Biology, University of Geneva, 30, quai Ernest-Ansermet, CH-1211, Geneva 4, Switzerland

    J. -M. Tiercy & R. Weil

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  1. J. -M. Tiercy
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  2. R. Weil
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Tiercy, J.M., Weil, R. Serum-induced stimulation of snRNA synthesis in mouse 3T3 fibroblasts. Experientia 41, 82–84 (1985). https://doi.org/10.1007/BF02005887

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