Summary
Aplysianin-A, an antibacterial and antineoplastic factor in the albumen gland of the sea hareAplysia kurodai, was isolated. It had a molecular weight of approximately 320 kD and consisted of subunits with a molecular weight of 85 kD. It contained 9.8% neutral sugar. Aplysianin A showed 50% inhibition ofBacillus subtilis growth at a concentration of 4 μg protein/ml and 50% lysis of murine MM46 tumor cells at 14 ng protein/ml. A partial identity of antigenic specificity of the purified specimen with an antineoplastic factor fromAplysia eggs was observed in immunodiffusion tests.
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Acknowledgment. We are indebted to the staff of Fisheries Research Laboratory, Faculty of Agriculture, University of Tokyo, Maisaka, for the collection of sea hares.
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Kamiya, H., Muramoto, K. & Yamazaki, M. Aplysianin-A, an antibacterial and antineoplastic glycoprotein in the albumen gland of a sea hare,Aplysia kurodai. Experientia 42, 1065–1067 (1986). https://doi.org/10.1007/BF01940736
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DOI: https://doi.org/10.1007/BF01940736