Skip to main content
SpringerLink
Log in

Thymine dimer repair in fibroblasts of patients with dysplastic naevus syndrome (DNS)

  • Short Communications
  • Current Activities in the Department of Research of the University Hospital, Basel
  • Published:
Experientia Aims and scope Submit manuscript

Summary

Dysplastic naevus syndrome (DNS) is frequently observed in association with familial melanoma and xeroderma pigmentosum (XP), but the role of UV-light in the development of DNS has not been elucidated. Previous work has shown that UV-induced unscheduled DNA synthesis is associated with the early loss of antigenicity observed in immunoassays using a monoclonal antibody specific for thymine-thymine dimers. We now show that the rate of loss of antigenicity, which reflects the relative amount of bound antibody, observed during the first 60 min following 10 Jm−2 UVC irradiation is significantly reduced (p=0.02) in cultures of fibroblasts from 7 out of 8 DNS patients compared with the results from cells of a group of 30 healthy volunteers. This observation suggests an early event in excision repair is altered in the majority of DNS patients.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Bale, S. J., Chakravati, A., and Greene, M. H., J. hum. Genet.38 (1986) 188.

    CAS  Google Scholar 

  2. Butterworth, T., and Ladda, R. L., Clinical Genodermatology, vol. 2, Light-sensitive genodermatoses, pp. 1–17. Praeger, New York 1981.

    Google Scholar 

  3. Cesarini, J. P., in: Familial Cancer, pp. 124–129. Eds Hj. Müller and W. Weber. Karger, Basel 1985.

    Google Scholar 

  4. Clarkson, J. M., Mitchell, D. L., and Adair, G. M., Mutat Res.112 (1983) 287.

    CAS  PubMed  Google Scholar 

  5. Davis, L. G., Dibner, M. D., and Battey, J. F. Basic Methods in Molecular Biology, pp. 41–50, 119–128. Elsevier, Amsterdam 1986.

    Google Scholar 

  6. Eggset, G., Volden, G., and Krokan, H., Carcinogenesis4 (1983) 745.

    Article  CAS  PubMed  Google Scholar 

  7. Elder, D. E., Goldman, L. I., Goldman, S. C., Greene, M. H., and Wallace, H. C., Cancer46 (1980) 1787.

    Article  CAS  PubMed  Google Scholar 

  8. Green, A. C., and O’Rourke, M. G. E., JNCI74 (1985) 977.

    CAS  PubMed  Google Scholar 

  9. Greene, M. H., Reimer, R. R., Clark, W. H., and Mastrangelo, M. J., Semin. Oncol.5 (1978) 85.

    CAS  PubMed  Google Scholar 

  10. Kraemer, K. H., and Greene, M. H., Dermat. Clin.3 (1985) 225.

    Article  CAS  Google Scholar 

  11. Maniatis, T., Fritsch, E. F., and Sambrook, J., Molecular Cloning. A Laboratory Manual, pp. 280–281, 438–439. Cold Springer Harbor Laboratory 1982.

  12. Paterson, M. C., Middlestadt, M. V., Mac Farlane, S. J., Gentner, N. E., and Weinfeld, M., J. cell. Sci.6, Suppl. (1987) 161.

    Article  CAS  Google Scholar 

  13. Patton, J. D., Rowan, L. A., Mendrala, A. L., Howell, J. N., Maher, V. M., and McCormick, J. J., Photochem Photobiol.39 (1983) 37.

    Article  Google Scholar 

  14. Penn, I., Immun. Today6 (1985) 206.

    Article  Google Scholar 

  15. Perera, M., Um, K., Greene, M. H., Walters, H. L., Bredberg, A., and Kraemer, K., Cancer Res.46 (1986) 1005.

    CAS  PubMed  Google Scholar 

  16. Peters, J. H., Baumgartner, H., and Schulze, M., Monoklonale Antikörper: Direkter und indirekter ELISA zum Nachweis vom monoklonalen Antikörpern gegen lösliche Antigene, pp. 122–128. Springer Verlag, Heidelberg 1985.

    Book  Google Scholar 

  17. Ramsey, R. G., Chen, P., and Imray, F. P. Cancer Res.42 (1982) 2909.

    Google Scholar 

  18. Reimer, R. R., Clark, W. H., Greene, M. H., Ainsworth, A. M., and Fraumeni, J. F., J. Am. med. Ass.239 (1978) 744.

    Article  CAS  Google Scholar 

  19. Roth, M., Mueller, H. J., and Boyle, J. M., Carcinogenesis8 (1987) in press.

  20. Sachs, L., Statistische Auswertungsmethoden. Springer Verlag, Heidelberg 1969.

    Google Scholar 

  21. Smith, P. J., Greene, M. H., and Devlin, D. A., Int. J. Cancer30 (1982) 39.

    Article  CAS  PubMed  Google Scholar 

  22. Strickland, P. T., and Boyle, J. M., Photochem. Photobiol.34 (1981) 595.

    Article  CAS  PubMed  Google Scholar 

  23. Tatsumi, K., Tuyoda, M., Hashimoto, T., Furuyama, J., Kurihara, T., Inoue, M., and Takebe, H., Carcinogenesis8 (1987) 53.

    Article  CAS  PubMed  Google Scholar 

  24. Teppo, L., Pakkanen, M., and Hakulinen, T., Cancer41 (1978) 2018.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Human Genetics, Dept of Research of the University Clinics, Kantonsspital, CH-4031, Basel

    M. Roth & Hj. Müller

  2. Dept of Human Genetics, University Children’s Hospital, CH-4005, Basel, Switzerland

    M. Roth & Hj. Müller

  3. Paterson Institute for Cancer Research, Christie Hospital and Holt Radium Institute, M20 9BX, Manchester, England

    J. M. Boyle

Authors
  1. M. Roth
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. M. Boyle
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hj. Müller
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Roth, M., Boyle, J.M. & Müller, H. Thymine dimer repair in fibroblasts of patients with dysplastic naevus syndrome (DNS). Experientia 44, 169–171 (1988). https://doi.org/10.1007/BF01952205

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01952205

Key words

Search

Navigation