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Changes in glycosaminoglycan sulfation and protein kinase C subcellular distribution during differentiation of the human colon tumor cell line Caco-2

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Summary

During the spontaneous differentiation (day 5 to day 15 of the culture) of Caco-2 cells, the sulfation of cell layer glycosaminoglycans increased, whereas protein kinase C activity was concomitantly redistributed from the membrane to the cytosol. The protein kinase C activators, 4β-phorbol 12β-myristate, 13α-acetate and 1,2-dioctanoyl-glycerol inhibited glycosaminoglycan sulfation. By contrast, 4α-phorbol 12, 13 didecanoate was ineffective.

These results suggest that membrane-bound PKC may exert a modulatory effect on glycosaminoglycan sulfation, and this effect is gradually attenuated as Caco-2 cell differentiation progresses.

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References

  1. Poole, A. R., Biochem. J.236 (1986) 1.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Gallagher, J. T., Lyon, M., and Steward, W. P., Biochem. J.236 (1986) 313.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Kolset, S. O., and Kjellen, L., Glycoconjugate J.3 (1986) 287.

    Article  CAS  Google Scholar 

  4. Carson, D. L., and Baxter, C. S., Biochem. biophys. Res. Commun.135 (1986) 909.

    Article  CAS  PubMed  Google Scholar 

  5. Castagna, M., Takai, Y., Kaibuchi, K., Sano, K., Kikkawa, U., and Nishizuka, Y., J. biol. Chem.257 (1982) 7847.

    Article  CAS  PubMed  Google Scholar 

  6. Rousset, M., Laburthe, M., Pinto, M., Chevalier, G., Rouyer-Fessard, C., Dussaulx, E., Trugnan, G., Boige, N., Brun, J. L., and Zweibaum, A., J. Cell Physiol.123 (1985) 377.

    Article  CAS  PubMed  Google Scholar 

  7. Grasset, E., Pinto, M., Dussaulx, E., Zweibaum, A., and Desjeux, J. F., Am. J. Physiol.247 (1984) C 260.

    Article  Google Scholar 

  8. Pinto, M., Robine-Léon, S., Appay, M.D., Kedinger, M., Triadou, N., Dussaulx, E., Lacroix, B., Simon-Assmann, P., Haffen, K., Fogh, J., and Zweibaum, A., Biol. cell,47 (1983) 323.

    Google Scholar 

  9. Lévy, P., Robert, A., and Picard, J., Biol. cell62 (1988) 255.

    Article  PubMed  Google Scholar 

  10. Fogh, J., Fogh, J. M., and Orfeo, T., J. natl Cancer Inst.59 (1977) 221.

    Article  CAS  PubMed  Google Scholar 

  11. Lévy, P., Picard, J., and Bruel, A., Life Sci.35 (1984) 2613.

    Article  PubMed  Google Scholar 

  12. Bitter, T., and Muir, H., Analyt. Biochem.4 (1962) 331.

    Article  Google Scholar 

  13. Cherqui, G., Caron, M., Wicek, D., Lascols, O., Capeau, J., and Picard, J., Endocrinology118 (1986) 1759.

    Article  CAS  PubMed  Google Scholar 

  14. Caron, M., Cherqui, G., Wicek, D., Capeau, J., Bertrand, J., and Picard, J., Experientia44 (1988) 34.

    Article  CAS  PubMed  Google Scholar 

  15. Hermelin, B., Cherqui, G., Bertrand, F., Paul, A., Garcia, I., and Picard, J., FEBS Lett.229 (1988) 161.

    Article  CAS  PubMed  Google Scholar 

  16. Skoglund, G., Hansson, A., and Ingelman-Sunberg, M., Acta chem. scand.39 (1985) 219.

    Article  CAS  Google Scholar 

  17. Simon-Assman, P., Bouzigues, F., Daviaud, D., Haffen, K., and Kedinger, M., Cancer Res.47 (1988) 4478.

    Google Scholar 

  18. Kapoor, R., and Prehm, P., Eur. J. Biochem.137 (1983) 589.

    Article  CAS  PubMed  Google Scholar 

  19. Kolset, S. O., Kjellen, L., Seljelid, R., and Lindahl, U., Biochem. J.210 (1983) 661.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Hutchison, C. J., and Yasin, R., Devl Biol.115 (1986) 78.

    Article  CAS  Google Scholar 

  21. Jalkanen, M., Medical Biol.65 (1987) 41.

    CAS  Google Scholar 

  22. Velasco, G., Iglesias, C. F., Dominguez, P., Barros, F., Gascon, S., and Lazo, P. S. Biochem. biophys. Res Commun.139 (1986) 875.

    Article  CAS  PubMed  Google Scholar 

  23. Kiss, Z., Deli, E., and Kuo, J. F., FEBS Lett.231 (1988) 41.

    Article  CAS  PubMed  Google Scholar 

  24. Kraft, A. S., and Anderson, W. N. B., Nature301 (1983) 621.

    Article  CAS  PubMed  Google Scholar 

  25. Issandou, M., and Darbon, J. M., Biochem. biophys. Res Commun.151 (1988) 458.

    Article  CAS  PubMed  Google Scholar 

  26. Hasumi, F., Tanji, N., and Mori, Y., J. Pharmacobiol. Dyn.10 (1987) 528.

    Article  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Laboratoire de Biochimie, INSERM, Faculté de Médecine Saint-Antoine, 27 rue Chaligny, F-75 571, Paris Cédex 12, France

    P. Levy, G. Cherqui, A. Robert, D. Wicek & J. Picard

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  1. P. Levy
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  2. G. Cherqui
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  3. A. Robert
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  4. D. Wicek
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  5. J. Picard
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Levy, P., Cherqui, G., Robert, A. et al. Changes in glycosaminoglycan sulfation and protein kinase C subcellular distribution during differentiation of the human colon tumor cell line Caco-2. Experientia 45, 588–591 (1989). https://doi.org/10.1007/BF01990515

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  • DOI: https://doi.org/10.1007/BF01990515

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