Skip to main content
SpringerLink
Log in

Dissociated cell suspensions ofCarcinus maenas Y-organs as a tool to study ecdysteroid production and its regulation

  • Research Articles
  • Published:
Experientia Aims and scope Submit manuscript

Abstract

In vitro ecdysteroid production by dissociated Y-organ cells of the shore crabCarcinus maenas was characterized during short-term incubations. Under optimized conditions (M199 adjusted to crab osmolality and with the addition of 10% foetal calf serum), ecdysteroid production by dispersed cells increased linearly during 4-hour incubations, with little intra-assay variation. 25-deoxyecdysone was mainly produced. PurifiedCarcinus molt inhbiting hormone (CamMIH) produced a dose-dependent inhibition of ecdysteroid production by dispersed cells. The cells were about 50 times more sensitive than whole glands to MIH. Other structurally-related peptides were tested.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Chang, E. S., and O'Connor, J. D., in: Endocrinology of Selected Invertebrate Types, p. 259. Eds H. Laufer and R. G. H. Downer. Alan R. Liss, Inc. New York 1988.

    Google Scholar 

  2. Spindler, K.-D., Keller, R., and O'Connor, J. D., in: Progress in Ecdysone Research, p. 247. Ed. J. A. Hoffman. Elsevier/North-Holland Biomedical Press, Amsterdam 1980.

    Google Scholar 

  3. Spaziani, E., in: Morphogenetic Hormones in Arthropods, vol. 2, p. 235. Ed. A. P. Gupta. Rutgers University Press, New Brunswick-London 1990.

    Google Scholar 

  4. Lachaise, F., Le Roux, A., Hubert, M., and Lafont, R., J. crust. Biol.13 (1993) 198.

    Article  Google Scholar 

  5. Smith, W. A., and Sedlmeier, D., Invert. Reprod. Develop.18 (1990) 77.

    Article  CAS  Google Scholar 

  6. Smith, W. A., Rountree, D. B., Bollenbacher, W. E., and Gilbert, L. I., in: Insect Neurochemistry and Neurophysiology, p. 319. Eds A. B. Borkovec and D. B. Gelman, The Humana Press, Clifton, New Jersey 1986.

    Google Scholar 

  7. Mattson, M. P., and Spaziani, E., J. exp. Zool.236 (1985) 93.

    Article  CAS  PubMed  Google Scholar 

  8. Webster, S. G., Gen. comp Endocr.61 (1986) 237.

    Article  CAS  PubMed  Google Scholar 

  9. Webster, S. G., and Keller, R., J. Comp. Physiol. B156 (1986) 617.

    Article  CAS  Google Scholar 

  10. Mattson, M. P., and Spaziani, E., Molec. cell. Endocr.42 (1985) 185.

    Article  CAS  PubMed  Google Scholar 

  11. Saïdi, B., Blais, C., de Bessé, N. and Lachaise, I., abstr. in: First European Crustacean Conference, Paris 31/08–05/09 (1992).

  12. Sedlmeier, D., and Fenrich, R., J. exp. Zool.265 (1993) 448.

    Article  CAS  Google Scholar 

  13. Von Gliscynski, U., and Sedlmeier, D., J. exp. Zool.265 (1993) 454.

    Article  Google Scholar 

  14. Böcking, D., Dauphin-Villemant, C., Sedlmeir, D., Blais, C., and Lafont, R., Insect Biochem. molec. Biol.23 (1993) 57.

    Article  Google Scholar 

  15. Lachaise, F., Hubert, M., Webster S. G., and Lafont, R., J. Insect Physiol.34 (1988) 557.

    Article  CAS  Google Scholar 

  16. Lachaise, F., Meister, M. F., Hétru, C. and Lafont, R., Mol. cell. Endocr.45 (1986) 253.

    Article  CAS  Google Scholar 

  17. Rudolph, P. H., and Spaziani, E., Gen. comp. Endocr.88 (1992) 235.

    Article  CAS  PubMed  Google Scholar 

  18. Drach, P., and Tchernigovtzeff, C., Vie Milieu18 (1967) 596.

    Google Scholar 

  19. Blais, C., and Lafont, R., Hoppe-Seyler's Z. physiol. Chem.365 (1984) 809.

    Article  CAS  Google Scholar 

  20. Webb, D. A., Proc. R. Soc.129 (1940) 107.

    CAS  Google Scholar 

  21. Chang, E. S., and O'Connor, J. D., in: Methods of Hormone Radioimmunoassay, p. 797. Eds B. M. Jaffe and L. R. Behrmann. Academic Press, New York 1979.

    Google Scholar 

  22. Chang, E. S., and O'Connor, J. D., Proc. natl Acad. Sci. USA74 (1977) 615.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. Hopkins, P. M., Gen. comp. Endocr.63 (1986) 362.

    Article  CAS  PubMed  Google Scholar 

  24. Hopkins, P. M., Am. Zool.32 (1992) 450.

    Article  CAS  Google Scholar 

  25. Lachaise, F., Carpentier, G., Sommé, G., Colardeau, J., and Beydon, P., J. exp. Zool.252 (1986) 283.

    Article  Google Scholar 

  26. Mattson, M. P., Zool. Sci.3 (1986) 733.

    CAS  Google Scholar 

  27. Naya, Y., Kishida, K., Sugiyama, M., Murata, M., Miki, W., Ohnishi, M., and Nakanishi, K. Experientia44 (1988) 50.

    Article  CAS  PubMed  Google Scholar 

  28. Schoettker, P. J., and Gist, D. H., J. crust. Biol.10 (1990) 487.

    Article  Google Scholar 

  29. Soumoff, C., and O'Connor, J. D., Gen. comp. Endocr.48 (1982) 432.

    Article  CAS  PubMed  Google Scholar 

  30. Blais, C., Sefiani, M., Toullec. J. Y., and Soyez, D., Invert. Reprod. Dev. (in press).

  31. Jegla, T. C., Rulland, C., Kegel, G., and Keller, R., J. comp. Physiol. B152 (1983) 91.

    Article  CAS  Google Scholar 

  32. Keller, R., and Schmid, E., J. comp. Physiol. B130 (1979) 347.

    Article  CAS  Google Scholar 

  33. Naya, Y., Ohnishi, M., Ikeda, M., Miki, W., and Nakanishi, K., Proc. natl Acad. Sci. USA86 (1989) 6826.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  34. Sonobe, H., Kamba, M., Ohta, K., Ikeda, M., and Naya, Y., Experientia47 (1991) 948.

    Article  CAS  Google Scholar 

  35. Chang, E. S., and Brody, M. D., Adv. Cell Culture7 (1989) 19.

    Article  CAS  Google Scholar 

  36. Webster, S. G., Proc R. Soc. Lond. B251 (1993) 53.

    Article  CAS  Google Scholar 

  37. Keller, R., Experientia48 (1992) 439.

    Article  CAS  PubMed  Google Scholar 

  38. Webster, S. G., Proc. R. Soc. Lond. B244 (1991) 247.

    Article  CAS  Google Scholar 

  39. Chang, E. S., Prestwich, G. D., and Bruce, M. J., Biochem. biophys. Res. Commun.171 (1990) 818.

    Article  CAS  PubMed  Google Scholar 

  40. Tensen, C. P., De Kleijn, D. P. V., and Van Herp, F., Eur. J. Biochem.200 (1991) 155.

    Article  Google Scholar 

  41. Soyez, D., Le Caer, J. P., Noël, P. Y., and Rossier, J., Neuropeptides20 (1991) 25.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratoire de Biochimie et Physiologie du Développement, CNRS URA 686, Ecole Normale Supérieure, 46 rue d'Ulm, F-75230, Paris cedex 05, France

    J. Y. Toullec & C. Dauphin-Villemant

Authors
  1. J. Y. Toullec
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C. Dauphin-Villemant
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Toullec, J.Y., Dauphin-Villemant, C. Dissociated cell suspensions ofCarcinus maenas Y-organs as a tool to study ecdysteroid production and its regulation. Experientia 50, 153–158 (1994). https://doi.org/10.1007/BF01984955

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01984955

Key words

Search

Navigation