Abstract
The L2/HNK-1 carbohydrate epitope has been shown to carry an unusual 3′-sulfoglucuronic acid linkedO-glycosidically through a neolactosyl-type back bone to a ceramide residue. Using monoclonal antibodies, the same or a closely related epitope has also been detectedN-glycosidically linked to glycoproteins, amongst them several neural cell adhesion molecules. We used synthetic glycolipids carrying sulfated or non-sulfated glucuronic acid attached to ceramide through glycans of different length to show that not only the sulfated glucuronic acid but also the neolactosyl-type backbone is essential for the recognition of the L2/HNK-1 carbohydrate by a monoclonal antibody, its binding to laminin and its role in neural cell migration and outgrowth of processes from neurons and astrocytes.
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Abbreviations
- mab:
-
monoclonal antibody
- TLC:
-
thin layer chromatography
- HRP:
-
horseradish peroxidase
- glcA:
-
glucuronic acid
- gal:
-
galactose
- glcNAc:
-
N-acetyl-glucosamine
- man:
-
mannose
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Schmitz, B., Schachner, M., Ito, Y. et al. Determination of structural elements of the L2/HNK-1 carbohydrate epitope required for its function. Glycoconjugate J 11, 345–352 (1994). https://doi.org/10.1007/BF00731208
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DOI: https://doi.org/10.1007/BF00731208