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Cell-cell interactions influence oligosaccharide modifications on mucins and other large glycoproteins

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Abstract

Intratumoral phenotypic diversity is well documented with regard to tumor associated carbohydrate antigens (TACA). The factors which control the expression of these cell-surface oligosaccharides on different cells of the same tumor are not understood. We investigated the expression of a panel of mucin associated oligosaccharides in cell lines growing at different surface densities (number of cells per cm2 of growth flask). Results show that the apparent expression of extended Lea-Lex, Lea and Lex, sialyl Lea, Tn and sialyl Tn varies with density of growth by an invasive human squamous cell lung carcinoma cell line (NU6-1), a benign variant (NE-18) and the human lung epithelial cell line BEAS-2B. The results indicate that one of the factors influencing the apparent expression of mucin-associated oligosaccharides is cell-cell interactions.

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Abbreviations

Mab:

monoclonal antibody

FIT:

fluorescein isothiocyanate

TACA:

tumor associated carbohydrate antigen

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Author information

Author notes

  1. Jennifer Laroe

    Present address: Colorado State University School of Veterinary Medicine, Fort Collins, CO, USA

  2. Alex Goldsmith

    Present address: Department of Internal Medicine, University of Colorado Health Sciences Center, 80262, Denver, CO, USA

Authors and Affiliations

  1. University of Colorado Cancer Center, 80262, Denver, CO, USA

    Patricia L. Stranahan, Jennifer Laroe, Ronda McCombs, Alex Goldsmith, Iffat Rahim, Mark Overland & David E. Pettijohn

  2. Department of Biochemistry, 80262, Denver, CO, USA

    Patricia L. Stranahan, Jennifer Laroe, Ronda McCombs, Alex Goldsmith, Iffat Rahim, Mark Overland & David E. Pettijohn

  3. Department of Biophysics and Genetics, 80262, Denver, CO, USA

    Patricia L. Stranahan, Jennifer Laroe, Ronda McCombs, Alex Goldsmith, Iffat Rahim, Mark Overland & David E. Pettijohn

  4. University of Colorado Health Sciences Center, 80262, Denver, CO, USA

    Patricia L. Stranahan, Jennifer Laroe, Ronda McCombs, Alex Goldsmith, Iffat Rahim, Mark Overland & David E. Pettijohn

Authors
  1. Patricia L. Stranahan
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  2. Jennifer Laroe
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  3. Ronda McCombs
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  4. Alex Goldsmith
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  5. Iffat Rahim
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  6. Mark Overland
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  7. David E. Pettijohn
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Stranahan, P.L., Laroe, J., McCombs, R. et al. Cell-cell interactions influence oligosaccharide modifications on mucins and other large glycoproteins. Glycoconjugate J 13, 741–747 (1996). https://doi.org/10.1007/BF00702338

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  • DOI: https://doi.org/10.1007/BF00702338

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