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Identification of a Novel Plant Virus Promoter using a Potyvirus Infectious Clone

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Abstract

A putative promoter from the strawberry vein banding caulimovirus (SVBV) genome was identified by its ability to drive infection with full-length cDNA of the zucchini yellow mosaic RNA potyvirus (ZYMV). A high rate of infection was obtained with the cDNA under control of the SVBV promoter using particle bombardment technology. The SVBV promoter shows 60% homology to the cauliflower mosaic virus 35S promoter in the domain spanning the conserved motifs of CCACT (at −83) and the TATA box (at −31), to the transcription start. The 3′-end one-third of the putative promoter (328 bp) was sufficient to invoke full infectivity with the ZYMV clone, and drove transient reporter gene expression in Solanaceae and Cucurbitaceae transformed with a binary plant transformation vector. Stable expression of a reporter gene (GUS) under control of the truncated SVBV promoter was shown in transformed tobacco shoots in roots, leaves and stems.

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Authors and Affiliations

  1. Departments of Virology, Agricultural Research Organization, The Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel

    Yongzeng Wang, Victor Gaba & Xiao-Di Xia

  2. Israel

    Dalia Wolf

Authors
  1. Yongzeng Wang
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  2. Victor Gaba
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  3. Dalia Wolf
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  4. Xiao-Di Xia
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  5. Amit Gal-On
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Wang, Y., Gaba, V., Wolf, D. et al. Identification of a Novel Plant Virus Promoter using a Potyvirus Infectious Clone. Virus Genes 20, 11–17 (2000). https://doi.org/10.1023/A:1008199805099

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