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Glycogen synthesis by cell-free extracts from the dimorphic yeast Candida albicans

  • Biochemistry
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Abstract

A particulate glucosyltransferase prepared from budding and filamentous cultures of Candida albicans used uridine diphosphate glucose as sole glucosyl donor in a reaction (measured by following the incorporation of [14C]-glucose from UDP [14C]-glucose into polymer) stimulated by glucose-6-phosphate and inhibited by adenosine triphosphate and guanosine triphosphate. The radiolabelled reaction product was solubilized by α-amylase, and, on oxidation with periodate followed by reduction with borohydride and acid hydrolysis, yielded erythritol and glycerol in the ratio of 4 to 1. The radiolabelled glucosyl residues were attached to an endogenous acceptor of high molecular weight.

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  1. Peter Orlean

    Present address: Institut für Botanik der Universität Regensburg, Universitätsstraße 31, 8400, Regensburg, Germany

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  1. Sub-department of Chemical Microbiology, Department of Biochemistry, University of Cambridge, Tennis Court Road, CB2 1QW, Cambridge, UK

    Peter Orlean

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  1. Peter Orlean
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Orlean, P. Glycogen synthesis by cell-free extracts from the dimorphic yeast Candida albicans . Antonie van Leeuwenhoek 50, 341–348 (1984). https://doi.org/10.1007/BF00394647

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  • DOI: https://doi.org/10.1007/BF00394647

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