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Reactions of reversion occuring with theTrichoderma reesei CL-847 cellulase system

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Summary

Poplar wood chips were pretreated by steam explosion and the cellulosic residue was hydrolysed with the cellulase complex ofTrichoderma reesei CL 847. The hydrolysate contains the β (1–6) disaccharide gentiobiose. Incubation of cellobiose with the same cellulase system led to the synthesis of Glc-Glc dimers which were characterized and quantified as gentiobiose, cellobiose, laminarabiose, sophorose and trehalose, whose origin was clarified by using carbon-13 glucose selectively labelled on C1. We propose a mechanism to explain these reversion reactions.

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Authors and Affiliations

  1. Centre de Recherches sur les Macromolécules Végétales C.N.R.S., B.P. 53X, 38041, Grenoble cedex, France

    B. Toussaint & M. R. Vignon

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  1. B. Toussaint
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  2. M. R. Vignon
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Toussaint, B., Vignon, M.R. Reactions of reversion occuring with theTrichoderma reesei CL-847 cellulase system. Biotechnol Lett 12, 587–592 (1990). https://doi.org/10.1007/BF01030757

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