Summary
Mucor miehei lipase has been adsorbed on Celite and covalently bound to nylon. The obtained derivatives have been studied regarding their ability for synthetize several flavouring esters in biphasic aqueous/organic media. The influence of the immobilization procedure on the synthetic activity of the derivatives was considered. Solvent hydrophobicity and water content in the biphasic system influenced both enzyme stability and equilibrium displacements. In this way, solvents with log P>3.5 and less than 1% water were optimal. It was important to consider pH effects on enzyme microenvironment when using acidic substrates. Optimum temperature and reuse of the catalyst were also checked.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bello M, Thomas D and Legoy M D (1987)Biochem Biophys Res Commun 146, 361–367
Carrea G (1984)Trends Biotechnol 2, 102–106
Dordick J S (1989)Enzyme Microb Technol 11, 194–211
Galfield I L (1986)Lebensm-Wiss Technol 19, 87–88
Gillies B, Yamazaki H and Armstrong D W (1987)Biotechnol Lett 9, 709–714
Goderis H L, Ampe G, Feiten M P, Fouwe B L, Guffens W M, Van Canwenberg S M and Tobbach P P (1987)Biotechnol Bioeng 30, 258–266
Gray L J, Narang J S and Baker S A (1990)Enzyme Microb Technol 12, 800–807
Hartree H (1972)Anal Biochem 48, 422–427
Hornby W E and Goldstein L (1976)Methods Enzymol 44, 118–134
Horvath C (1974)Biochim Biophys Acta 358, 164–177
Inada Y, Nishimura H, Takahashi K, Yoshimoto T, Saha A R and Saito Y (1984)Biochem Biophys Res Commun 122, 845–850
Kilara A (1985)Process Biochem 20, 35–45
Kimura Y, Tanaka A, Sonomoto K, Nihira T and Fukui S (1983)Eur J Appl Microbiol Biotechnol 17, 107–112
Klibanov A M (1986)Chem Tech June, 354–359
Laane C, Boeren S and Vos K (1984)Trends Biotechnol 3, 251–252
Laane C, Boeren S, Vos K and Veeger C (1987)Biotechnol Bioeng 30, 81–87
Langrand G, Rondot N, Triantaphylides C and Baratti J (1990)Biotechnol Lett 12, 581–586
Lavayre J and Baratti J (1982)Biotechnol Bioeng 24, 1007–1013
Lieberman R B and Ollis D F (1975)Biotechnol Bioeng 17, 1401–1419
Lozano P, Manjón A, Romojaro F and Iborra J L (1988)Process Biochem 23, 75–78
Marlot C, Langrand G, Triantaphylides C and Baratti J (1985)Biotechnol Lett 7, 647–650
Mitchell J and Smith D M (1980) inAquametry (2nd ed) Part III. Wiley-Interscience Publishers, New York
Posorske L H (1984)J Amer Oil Chem Soc 61, 1758–1760
Ratledge C (1984)Fete Seifen Austrichmittel 10, 379–389
Takahashi K, Kodera Y, Yoshimoto T, Ajima A, Matsushima A and Inada I (1985)Biochem Biophys Res Commun 131, 532–536
Takashi K, Tamaura Y, Kodera Y, Mihama T, Saito Y and Inada Y (1987)Biochem Biophys Res Commun 142, 291–296
Thompson R Q, Mandoke C S and Womack J P (1985)Anal Lett 18, 93–107
Ward C P (1985)Comprehensive Biotechnology (M Moo-Young Ed), Vol 3, pp 830–832. Pergamon Press, UK.
Welsh F W, Murray W D and Williams R E (1989)CRC Crit Rev Biotechnol 9, 105–169
Welsh F W and Williams R E (1990)Enzyme Microb Technol 12, 743–748
Yokozeki K, Yamanaka S, Takinami K, Hirose Y, Tanaka A, Sonomoto K and Fukui S (1982)Eur J Appl Microbiol Biotechnol 14, 1–5
Zaks A and Klibanov A M (1984)Science 224, 1249–1251
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Manjón, A., Iborra, J.L. & Arocas, A. Short-chain flavour ester synthesis by immobilized lipase in organic media. Biotechnol Lett 13, 339–344 (1991). https://doi.org/10.1007/BF01027679
Issue Date:
DOI: https://doi.org/10.1007/BF01027679