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Thermostability of α-chymotrypsin in water/organic solvent systems

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Summary

The influence of pH, temperature, substrate concentration and organic solvents (dimethylformamide, dimethylsulfoxide) on the α-chymotrypsin stability in a water/organic solvent system was studied. The enzyme activity was measured as the dipeptide, AcPheLeuNH2 synthesis and the ester substrate hydrolysis. Enzyme stability was enhanced by lower pH and temperature values and higher substrate concentrations. Dimethylsulfoxide allowed an higher enzyme stability than dimethylformamide. α-Chymotrypsin displayed an higher stability in the water medium when it was compared to the organic system.

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Authors and Affiliations

  1. Laboratório de Engenharia Bioquímica, Instituto Superior Técnico, 1000, Lisboa, Portugal

    M. I. Vidal, M. L. M. Serralheiro & J. M. S. Cabral

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  1. M. I. Vidal
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  2. M. L. M. Serralheiro
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  3. J. M. S. Cabral
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Vidal, M.I., Serralheiro, M.L.M. & Cabral, J.M.S. Thermostability of α-chymotrypsin in water/organic solvent systems. Biotechnol Lett 14, 1041–1044 (1992). https://doi.org/10.1007/BF01021055

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  • DOI: https://doi.org/10.1007/BF01021055

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