Summary
Human lipocortin (annexin) 1, a member of the annexin family of phospholipid binding proteins, has previously been expressed in E. coli (Huh et al., 1990). To improve the expression level of lipocortin 1 in E. coli, several expression vectors containing either the PL or the Ptrc promoter were constructed. The highest expression level, up to 20 % of the total E. coli proteins, was obtained with plasmid pHT3. Plasmid pHT3 contains the pUC origin, the lipocortin 1 cDNA under Ptrc promoter, and the lacI gene.
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Huh, KR., Cho, E.H., Lee, S.O. et al. High level expression of human lipocortin (annexin) 1 in Escherichia coli . Biotechnol Lett 18, 163–168 (1996). https://doi.org/10.1007/BF00128673
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DOI: https://doi.org/10.1007/BF00128673