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Effect of sugar chain structure on in vivo biological activity of a recombinant human glycoprotein expressed in SP2/0-AG14 cells

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Abstract

We have reported previously that recombinant human erythropoietin (rhEPO) produced from Sp2/0-Ag14 transformant had a low biological activity in vivo as compared with other human EPOs. rhEPO from SP2/0 has now been found to have a lower amount of sialic acid and different sugar chains from other hEPOs. This recombinant molecule contains disialobranches as major and complex sugar chains which showed broad peaks on gel-filtration chromatography. These differences may be responsible for its low in vivo bioactivity.

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Authors and Affiliations

  1. Department of Biological and Chemical Engineering, Faculty of Engineering, Gunma University Kiryu, Gunma, 376, Japan

    Shinji Sugaya, Hideya Ohashi & Takao Shinozawa

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  1. Shinji Sugaya
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  2. Hideya Ohashi
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  3. Takao Shinozawa
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Sugaya, S., Ohashi, H. & Shinozawa, T. Effect of sugar chain structure on in vivo biological activity of a recombinant human glycoprotein expressed in SP2/0-AG14 cells . Biotechnology Letters 19, 281–284 (1997). https://doi.org/10.1023/A:1018322127540

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  • DOI: https://doi.org/10.1023/A:1018322127540

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