Abstract
Selective enzymic esterification of free fatty acids, obtained from blackcurrant oil by chemical saponification, with n-butanol using four immobilized lipases under microwave irradiation and under classical heating was studied. A positive effect of microwave irradiation on chemical yields of the products of the enzymic reactions and specificity of lipases were observed in comparison with a controlled heating in an incubator equipped with shaking (classical heating) applied during the identical enzyme-mediated processes. The maximum quantity of γ-linolenic acid (30%) was obtained with Lipozyme used as biocatalyst of the reaction under microwave irradiation. The maximum quantity of butyl γ-linolenate (20%) was obtained by a Pseudomonas cepacia lipase catalyzed esterification under classical heating.
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Vacek, M., Zarevúcka, M., Wimmer, Z. et al. Selective enzymic esterification of free fatty acids with n-butanol under microwave irradiation and under classical heating. Biotechnology Letters 22, 1565–1570 (2000). https://doi.org/10.1023/A:1005637018167
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DOI: https://doi.org/10.1023/A:1005637018167