biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 43:559-562, 2000 | DOI: 10.1023/A:1002821715719

The Role of Peroxidase and Polyphenol Oxidase Isozymes in Wheat Resistance to Alternaria triticina

M. Tyagi1, Arvind M. Kayastha1,*, B. Sinha2
1 School of Biotechnology, Faculty of Science, Banaras Hindu University, Varanasi -, India
2 Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi -, India

Polyphenol oxidase activity was higher in resistant wheat cultivar ACC-8226 than in susceptible cultivar MP-845 in control sets and after inoculation of Alternaria triticina. However, similar polyphenol oxidase isozyme pattern was found in control and inoculated sets of both the cultivars, but the band intensity was higher after inoculation. Three and four peroxidase isozymes were found in ACC-8226 and MP-845, respectively. An extra peroxidase isozyme band was observed in both the cultivars after inoculation. The results suggest an active role of peroxidase and polyphenol oxidase in defence mechanism of wheat plants.

Keywords: phenolics; susceptibility; Triticum aestivum
Subjects: Alternaria triticina; peroxidase isozymes, resistance to Alternaria; phenolics, resistance to Alternaria triticina; polyphenol oxidase isozymes, resistance to Alternaria; susceptibility to Alternaria triticina; Triticum aestivum; wheat, resistance to Alternaria triticina

Published: December 1, 2000  Show citation

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Tyagi, M., Kayastha, A.M., & Sinha, B. (2000). The Role of Peroxidase and Polyphenol Oxidase Isozymes in Wheat Resistance to Alternaria triticina. Biologia plantarum43(4), 559-562. doi: 10.1023/A:1002821715719
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References

  1. Bashan, Y., Okon, Y., Henis, Y.: Peroxidase, polyphenol oxidase and phenols in relation to resistance against Pseudomonas syrigae pv. tomato in tomato plants.-Can. J. Bot. 65: 366-372, 1987. Go to original source...
  2. Ganguly, S., Dasgupta, D.R.: Characterization of polyphenol oxidase from susceptible and resistant varieties of tomato inoculated with the root knot nematode, Meloidogyne incognita.-Indian J. Nematol. 12: 320-329, 1982.
  3. Gross, G.G.: The biochemistry of lignification.-Adv. Bot. Res. 8: 25-63, 1980. Go to original source...
  4. Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the folin-phenol reagent.-J. biol. Chem. 193: 265-275, 1951. Go to original source...
  5. Martyn, R.D., Samuelson, D.A., Freeman, T.E.: Ultrastructural localisation of polyphenol oxidase activity in leaves of healthy and diseased water hyacinth.-Phytopathology 69: 1278-1287, 1979. Go to original source...
  6. Mathur, N., Vyas, A.: Changes in isozyme patterns of peroxidase and polyphenol oxidase by VAM fungi in roots of Ziziphus species.-J. Plant Physiol. 145: 498-500, 1995. Go to original source...
  7. Mayer, A.M., Harel, E.: Polyphenol oxidase in plants.-Phytochemistry 18: 193-215, 1979. Go to original source...
  8. Retig, N.: Changes in peroxidase and polyphenol oxidase associated with natural and induced resistance of tomato to Fusarium wilt.-Physiol. Plant Pathol. 4: 145-150, 1974. Go to original source...
  9. Seevers, P.M., Daly, J.M., Catedral, F.F.: The role of peroxidase isozymes in resistance to wheat stem rust disease.-Plant Physiol. 48: 353-360, 1971. Go to original source...
  10. Stafford, H.A., Galston, A.W.: Ontogeny and hormonal control of polyphenol oxidase isozymes in tobacco pith.-Plant Physiol. 46: 763-767, 1970. Go to original source...
  11. Tyagi, M., Kayastha, A.M., Sinha, B.: The role of phenolics and peroxidase in resistance to Alternaria triticina in bread wheat (Triticum aestivum L.)-J. Agron. Crop Sci. 181: 29-34, 1998. Go to original source...
  12. Vance, C.P., Anderson, J.O., Sherwood, R.T.: Soluble and cell wall peroxidases in reed canarygrass in relation to disease resistance and localized lignin formation.-Plant Physiol. 57: 920-922, 1976. Go to original source...
  13. Wojtaszek, P.: Oxidative burst: an early plant response to pathogen infection.-Biochem. J. 322: 681-692, 1997. Go to original source...