Skip to main content

Advertisement

SpringerLink
Log in

Regulation of desmosomal cell adhesion in human tumour cells by polyunsaturated fatty acids

  • Published:
Clinical & Experimental Metastasis Aims and scope Submit manuscript

Abstract

Desmosomes are key structures in cell-cell adhesion. In this study we examined the effect of n-6 essential fatty acids on the expression of desmoglein (Dsg), desmosomal cadherin and the formation of desmosomes in E-cadherin negative human breast, colon and lung cancer cells and melanoma cells. Electron microscopy revealed that cells cultured with gamma linolenic acid (GLA) showed increased cell-cell adhesion together with an increase in the formation of desmoglein-containing desmosomes. Western blotting studies of cellular proteins demonstrated that, following culture with fatty acids, Dsg expression was modified, with the greatest increase seen after GLA treatment. Other fatty acids increased Dsg expression, but to a lesser extent. It is concluded that GLA regulates desmosome-mediated cell-cell adhesion in human cancer cells, particularly in cells without E-cadherin.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Begin ME, Ells G, Das UN and Horrobin DF, 1986, Differential killing of human carcinoma cells supplemented with n-3 and n-6 polyunsaturated fatty acids. J Natl Cancer Inst, 77, 1053–62.

    Google Scholar 

  2. Horrobin DF, 1990, Essential fatty acids, lipid peroxidation, and cancer. In: Horrobin DF, ed. Omega-6 Essential Fatty Acids. New York: Wiley-Liss, pp. 351–78.

    Google Scholar 

  3. Begin ME, Ells G, Das UN and Horrobin DF, 1988, Polyunsaturated fatty acids induced cytotoxicity against tumor cells and its relationship to lipid peroxidation. J Natl Cancer Inst, 80, 188–94.

    Google Scholar 

  4. Hayashi Y, Fukushima S, Kishimoto S, et al.1992, Anticancer effects of free polyunsaturated fatty acids in an oily lymphographic agent following intrahepatic arterial administration to a rabbit bearing VX-2 tumor. Cancer Res, 52, 400–5.

    Google Scholar 

  5. Newman MJ, 1990, Inhibition of carcinoma and melanoma cell growth by type 1 transforming growth factor beta is dependent on the presence of polyunsaturated fatty acids. Proc Natl Acad Sci USA, 87, 5543–7.

    Google Scholar 

  6. Puntis MCA and Jiang WG, 1994, Effects of gamma linolenic acid and lithium linolinate on human monocytes and hepatoma cells. New Approaches to Cancer Treatment: unsaturated lipids and photodynamic therapy.In Horrobin DF, ed, Churchill-Livinstone. pp 40–67.

  7. Jiang WG, Hiscox S, Hallett MB, Scott C, Horrobin DF and Puntis MCA, 1995, Inhibition of hepatocyte growth factor/scatter factor induced motility and invasion of human cancer cells by gamma linolenic acid. Br J Cancer, 71, 744–52.

    Google Scholar 

  8. Jiang WG, Hiscox S, Hallett MB, Horrobin DF, Mansel RE and Puntis MCA, 1995, Regulation of the expression of E-cadherin in human colon cancer cells by gamma linolenic acid. Cancer Res, 55, 5043–8.

    Google Scholar 

  9. Jiang WG, 1996, E-cadherin and its associated protein catenin, cancer invasion and metastasis.Br J Surgery, 83, 437–46.

    Google Scholar 

  10. Koch PJ and Franke WW, 1994, Desmosomal cadherins, another growing multigene family of adhesion molecules. Curr Opinion Cell Biol, 6, 682–7.

    Google Scholar 

  11. Wheeler GN, Buxton RS, Parker AE, et al.1991, Desmosomal glycoproteins I, II and III: novel members of the cadherin superfamily. Biochem Soc Trans, 19, 1060–4.

    Google Scholar 

  12. Schmidt A, Heid HW, Schafer S, Nuber UA, Zimbelmann R and Franke WW, 1994, Desmosomes and cytoskeletal architecture in epithelial differentiation: cell type specific plaque components and intermediate filament anchorage. Eur J Cell Biol, 65, 229–45.

    Google Scholar 

  13. Legan PK, Collins JE and Garrod DR, 1992, The molecular biology of desmosomes and hemidesmosomes: what's in a name? BioEssays, 14, 385–93.

    Google Scholar 

  14. Schwartz MA, Owaribe K, Kartenbeck J and Franke WW, 1990, Desmosomes and hemidesmosomes: constitutive molecular components. Ann Rev Cell Biol, 6, 461–91.

    Google Scholar 

  15. Garrod DR, 1995, Desmosomes and cancer. Cancer Surveys, 24, 97–111.

    Google Scholar 

  16. Garrod DR, 1993, Desmosomes and hemidesmosomes. Curr Opinion Cell Biol, 5, 30–40.

    Google Scholar 

  17. Witcher LL, Collins R, Puttagunta S, et al.1996, Desmosomal cadherin binding domains of plakoglobin. J Biol Chem, 271, 10904–9.

    Google Scholar 

  18. Kowalczyk AP, Stappenbeck TS, Parry DAD, et al.1994, Structure and function of desmosomal transmembrane core and plaque molecules. Biophys Chem, 50, 97–112.

    Google Scholar 

  19. Sheu HM, Kitajima Y and Yaoita H, 1989, Involvement of protein kinase C in translocation of desmoplakins from cytosol to plasma membrane during desmosome formation in human squamous cell carcinoma cells grown in low to normal calcium concentration. Exp Cell Res, 185, 176–90.

    Google Scholar 

  20. Pasdar M, Li Z and Chan H, 1995, Desmosome assembly and disassembly are regulated by reversible protein phosphorylation in cultured epithelial cells. Cell Motility Cytoskeleton, 30, 108–21.

    Google Scholar 

  21. Amino K, Takahashi M, Honda Y and Fujimoto T, 1996, Tyrosine phosphorylation in desmosomes and hemidesmosomes of the corneal epithelium. J Histochem Cytochem, 44, 19–25.

    Google Scholar 

  22. Demlehner MP, Schafer S, Grund C and Franke WW, 1995, Continual assembly of half desmosomal structures in the absence of cell contacts and their frus-trated endocytosis: a coordinated sisyphus cycle. J Cell Biol, 131, 745–60.

    Google Scholar 

  23. Pauli BU, Cohen SM, Alroy J and Weinstein RS, 1978, Desmosome ultrastructure and the biological behaviour of chemical carcinogen induced urinary bladder carcinomas. Cancer Res, 38, 3276–85.

    Google Scholar 

  24. Alroy J, Pauli BU and Weinstein RS, 1981, Correlation between numbers of desmosomes and the aggressiveness of transitional cell carcinoma in the human urinary bladder. Cancer, 47, 104–12.

    Google Scholar 

  25. Green KJ, Stappenbeck TS, Noguchi S, Oyasu R and Nilles LA, 1991, Desmoplakin expression and distribution in cultured rat bladder epithelial cells of varying tumorigenic potential. Exp Cell Res, 193, 134–43.

    Google Scholar 

  26. Kocher O, Amaudruz AM and Gabbiani G, 1981, Desmosomes and gap junctions in percarcinomatous and carcinomatous conditions of squamous epithelial. J Submicroscopic Cytol, 13, 267–81.

    Google Scholar 

  27. Schindler AM, Amaudruz MA, Kocher O, Riotton G and Gabbiani G, 1981, Desmosomes and gap junctions in various epidermoid preneoplastic and neoplastic lesions of the cervix uteri. Acta Cytologica, 26, 466–70.

    Google Scholar 

  28. Stein LS, Stein DWJ, Echols J and Burghardt RC, 1993, Concomitant alterations of desmosomes, adhesiveness, and diffusion through gap junction channels in a rat ovarian transformation model system. Exp Cell Res, 207, 19–32.

    Google Scholar 

  29. Wright-Perkins S, Daniel MR and Walker C, 1995, Changes in expression of neural cell adhesion molecule and desmoplakin associated with phenotypic transitions in cloned cell lines from a non small cell lung carcinoma. Int J Oncol, 6, 627–33.

    Google Scholar 

  30. Natsugoe S, Sagara M, Shimada M, et al.1995, Expression of desmoglein-i cell-adhesion molecule in primary tumors and metastatic lymph-nodes of esophageal cancer. Int J Oncol, 6, 345–8.

    Google Scholar 

  31. Natsugoe S, Aikou T, Shimada M, et al.1994, Expression of desmoglein-i in squamous-cell carcinoma of the oesophagus. J Surg Oncol, 57, 105–10.

    Google Scholar 

  32. Imai K, Kumagai S, Nakagawa K, Yamamoto E, Nakanishi I and Okada Y, 1995, Immunolocalization of desmoglein and intermediate filaments in human oral squamous-cell carcinomas. Head Neck-J Sci Specialties Head Neck, 17, 204–12.

    Google Scholar 

  33. Conn IG, Vilela MJ, Garrod DR, Crocker J and Wallace D, 1990, Immuno-histochemical staining with monoclonal antibody-32-2b to desmosomal glycoprotein-1-its role in the histological assessment of urothelial carcinomas. Br J Urol, 65, 176–80.

    Google Scholar 

  34. Harada T, Shinohara M, Nakamura S, Shimada M and Oka M, 1992, Immunohistochemical detection of desmosomes in oral squamous-cell carcinomas-correlation with differentiation, mode of invasion, and metastatic potential. Int J Oral Maxillofacial Surg, 21, 346–9.

    Google Scholar 

  35. Hiraki A, Shinohara M, Ikebe T, Nakamura S, Kurahara S and Garrod DR, 1996, Immunohistochemical staining of desmosomal components in oral squamous-cell carcinomas and its association with tumor behavior. Br J Cancer, 73, 1491–7.

    Google Scholar 

  36. Albini A, Iwamoto Y, Kleinman HK, et al.1987, A rapid in vitroassay for quantitating the invasive potential of tumor cells. Cancer Res, 47, 3239–45.

    Google Scholar 

  37. Parish CR, Jakobsen KB and Coombe DR, 1992, A basement membrane permeability assay which correlates with the metastatic potential of tumor cells. Int J Cancer, 52, 378–83.

    Google Scholar 

  38. Singharao SK, Neal SW and Newman GR, 1993, Corpora amulacea could be an indicator of neurodegeneration. Neuropathol Appl Neurobiol, 19, 269–76.

    Google Scholar 

  39. Nei H, Saito T, Tobioka H, Itoh E, Mori M and Kudo R, 1996, Expression of component desmosomal proteins in uterine endometrial carcinoma and their relation to cellular differentiation. Cancer, 78 ,461–70.

    Google Scholar 

  40. Fearon KCH, Falconer JS, Ross JA, et al.1996, An open-label phase i/ii dose-escalation study of the treatment of pancreatic-cancer using lithium gammalinolenate. Anticancer Res, 16, 867–74.

    Google Scholar 

  41. Jiang WG, Bryce RP, Horrobin DF, Mansel RE, 1997, Gamma linolenic acid regulates gap junction communications in endothelial cells and their interaction with tumour cells. Prostaglandins Leukotrienes Essential Fatty Acids, 56, 307–16.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Metastasis Research Group, University Department of Surgery University of Wales College of Medicine, Cardiff

    Wen G. Jiang, Stephen Hiscox, Maurice B. Hallett, Malcolm C. A. Puntis & Robert E. Mansel

  2. EM Unit University of Wales College of Medicine, Cardiff

    Sim K. Singhrao

  3. Scotia Pharmaceuticals Ltd, Stirling, Scotland UK

    Richard P. Bryce & David F. Horrobin

Authors
  1. Wen G. Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sim K. Singhrao
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Stephen Hiscox
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Maurice B. Hallett
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Richard P. Bryce
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. David F. Horrobin
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Malcolm C. A. Puntis
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Robert E. Mansel
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jiang, W.G., Singhrao, S.K., Hiscox, S. et al. Regulation of desmosomal cell adhesion in human tumour cells by polyunsaturated fatty acids. Clin Exp Metastasis 15, 593–602 (1997). https://doi.org/10.1023/A:1018435229087

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1018435229087

Search

Navigation