Summary
It has been suggested that c-myc, one of the proto-oncogenes, plays a role in normal somatic cell proliferation and differentiation. To define whether c-myc is only expressed during somatic cell division or is also expressed during meiotic cell division, the production of c-myc mRNA and protein were investigated in the mouse testis by usingin situ hybridization with non-radioactive DNA probes and enzyme immunohistochemistry respectively. Forin situ hybridization, T-T dimerized DNA probes were used and DNAs hybridizedin situ were detected immunohistochemically using specific antibody against T-T dimer. The results indicate that c-myc mRNA and protein are expressed in a cell-cycle-dependent manner only in spermatogonia and not in spermatocytes and spermatids.
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Koji, T., Izumi, S., Tanno, M. et al. Localizationin situ ofc-myc mRNA andc-myc protein in adult mouse testis. Histochem J 20, 551–557 (1988). https://doi.org/10.1007/BF01002609
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DOI: https://doi.org/10.1007/BF01002609