Summary
Sulphatides have been studied by histochemical and biochemical procedures in the oviduct of the frog in different experimental conditions. In ovariectomized or hypophysectomized, animals, compared to sham-operated, an increase in sulphatides was observed. The progesterone treatment did not significantly, modify this lipid fraction in ovariectomized frogs, while in hypophysectomized frogs it induced a further increase. Densitographic profiles of the sulphatides, obtained by thinlayer chromatography (TLC) and also recorded by Tesak equipment, were similar in ovariectomized or hypophysectomized frogs following hormone treatment because they showed three distinct fractions in both experimental groups of animals. The appearance of a third fraction never previously observed was probably induced by the progesterone treatment. Moreover, under the effects of this hormone, the phospholipid fractions (phosphatidylcholine and phosphatidylethanolamine) also showed different densitographic profiles.
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References
Andreozzi, G., Botte, V. &Chieffi, G. (1971) L'ovidutto diRana esculenta nel corso del ciclo riproduttivo. Ricerche istologiche ed istochimiche.Boll. Zool. 38, 261–72.
Berra, B., di Palma, S. &Zambotti, F. (1974) Analisi cromatografica dei lipidi.,J. Res. Lab. Med. 1, 45–7.
Bolognani, L., Masserini, M., Bodini, P. A. Bolognani-fantin, A. M. &Ottaviani, E. (1981) Lipid composition in ganglia of Mollusca.,J. Neurochem. 36, 821–5.
Coles, L., Hay, J. B. &Gray, G. M. (1970) Factors affecting the glycosphingolipids composition of murine tissue.J. Lip. Res. 11, 158–67.
Dulaney, J. T. &Moser, H. W. (1977) In:Practical Enzymology of the Sphingolipidoses (edited byGlew, R. W. &Peters, S. P.), pp. 137–71. New York: Alan R. Liss.
Elison, R. P. (1971) Fertilization of frog body-cavity eggs:Rana pipiens eggs andRana clamitans sperm.Biol. Reprod. 8, 362–8.
Greven, H. (1980) Licht-und elektronenmikroskopische Untersuchungen zur Struktur und Histochemie des Oviduktepithels (Pars recta and pars convoluta I, II III) vonSalamandra salamandra (L) (Amphibia, Urodela).Z. mikrosk.-anat. Forsh. 94, 387–429.
Hirsch, P. &Peiffer, I. (1967) In:Cerebral Lipidoses (edited byCumings, J. N.), pp. 68. Oxford: Blackwell.
Kean, E. L. (1968) Rapid sensitive spectrophotometric method for quantitative determination of sulphatides.J. Lipid Res. 9, 319–27.
Mclaughlin, E. E. &Humphries jr, A. A. (1978) The jelly envelopes and fertilization of eggs of the newt,Notophtalmus viridescens.J. Morph. 158, 73–90.
Moreno, A. R. (1972) Histomorfologia del oviducto deBufo arenarum Hensel.Rev. Agronmoica del Noroeste Argentino 9, 585–602.
Picheral, B. (1977) La fecondation chez le triton Pleurodéle I. La traversée des enveloppes de l'oeuf par les spermatozoides.J. Ultrastruct. Res. 60, 106–20.
Shivers, C. A. &James, J. M. (1971) Morphology and histochemistry of the oviduct and egg-jelly layers in the frogRana pipiens.Anat. Rec. 166, 541–56.
Vitaioli, L., Baldoni, E., Fioretti, E., Dubois, G. &Bolognani, L. (1985) Fluctuations in arylsulphatase activity in the rabbit endometrium during the sexual cycle.Histochemistry 83, 127–31.
Vitaioli, L., Baldoni, E. &Bellini, L. (1986) Arylsulphatase activity in the frog oviduct during the recovery phase of the reproductive cycle.Cell. Mol. Biol. 32, 187–93.
Vitaioli, L., Baldoni, E., Bellini, L., Pederzoli, A. &Bolognani, L., (1987a) Arylsulphatase activity and cerebroside sulphates in the frog oviduct during the reproductive cycle.Histochem. J. 19, 85–93.
Vitaioli, L., Baldoni, E. &Bellini, L. (1987b) Arylsulphatase activity in the oviduct of the frogRana esculenta. I. Oestradiol-induced changes following ovariectomy and hypophysectomy.Histochem. J. 19, 217–24.
Vitaioli, L., Baldoni, E., Bellini, L., Pederzoli, A. &Bolognani, L. (1988a) Sulphatides in the frog oviduct: response to estradiol stimulation.Cell. Mol. Biol. 34, 373–82.
Vitaioli, L., Baldoni, E., Gobbetti, A. &Bellini, L. (1988b) Arylsulphatase activity in the oviduct of the frogRana esculenta. II. Progesterone-induced changes following ovariectomy and hypophysectomy.Histochem. J. 20, 301–7.
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Vitaioli, L., Baldoni, E., Bellini, L. et al. Changes induced by progesterone treatment in the sulphatides of the frog oviduct. Histochem J 22, 192–196 (1990). https://doi.org/10.1007/BF02386004
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DOI: https://doi.org/10.1007/BF02386004