Skip to main content
Log in

Changes induced by progesterone treatment in the sulphatides of the frog oviduct

  • Papers
  • Published:
The Histochemical Journal Aims and scope Submit manuscript

Summary

Sulphatides have been studied by histochemical and biochemical procedures in the oviduct of the frog in different experimental conditions. In ovariectomized or hypophysectomized, animals, compared to sham-operated, an increase in sulphatides was observed. The progesterone treatment did not significantly, modify this lipid fraction in ovariectomized frogs, while in hypophysectomized frogs it induced a further increase. Densitographic profiles of the sulphatides, obtained by thinlayer chromatography (TLC) and also recorded by Tesak equipment, were similar in ovariectomized or hypophysectomized frogs following hormone treatment because they showed three distinct fractions in both experimental groups of animals. The appearance of a third fraction never previously observed was probably induced by the progesterone treatment. Moreover, under the effects of this hormone, the phospholipid fractions (phosphatidylcholine and phosphatidylethanolamine) also showed different densitographic profiles.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Andreozzi, G., Botte, V. &Chieffi, G. (1971) L'ovidutto diRana esculenta nel corso del ciclo riproduttivo. Ricerche istologiche ed istochimiche.Boll. Zool. 38, 261–72.

    Google Scholar 

  • Berra, B., di Palma, S. &Zambotti, F. (1974) Analisi cromatografica dei lipidi.,J. Res. Lab. Med. 1, 45–7.

    Google Scholar 

  • Bolognani, L., Masserini, M., Bodini, P. A. Bolognani-fantin, A. M. &Ottaviani, E. (1981) Lipid composition in ganglia of Mollusca.,J. Neurochem. 36, 821–5.

    CAS  PubMed  Google Scholar 

  • Coles, L., Hay, J. B. &Gray, G. M. (1970) Factors affecting the glycosphingolipids composition of murine tissue.J. Lip. Res. 11, 158–67.

    CAS  Google Scholar 

  • Dulaney, J. T. &Moser, H. W. (1977) In:Practical Enzymology of the Sphingolipidoses (edited byGlew, R. W. &Peters, S. P.), pp. 137–71. New York: Alan R. Liss.

    Google Scholar 

  • Elison, R. P. (1971) Fertilization of frog body-cavity eggs:Rana pipiens eggs andRana clamitans sperm.Biol. Reprod. 8, 362–8.

    Google Scholar 

  • Greven, H. (1980) Licht-und elektronenmikroskopische Untersuchungen zur Struktur und Histochemie des Oviduktepithels (Pars recta and pars convoluta I, II III) vonSalamandra salamandra (L) (Amphibia, Urodela).Z. mikrosk.-anat. Forsh. 94, 387–429.

    CAS  Google Scholar 

  • Hirsch, P. &Peiffer, I. (1967) In:Cerebral Lipidoses (edited byCumings, J. N.), pp. 68. Oxford: Blackwell.

    Google Scholar 

  • Kean, E. L. (1968) Rapid sensitive spectrophotometric method for quantitative determination of sulphatides.J. Lipid Res. 9, 319–27.

    CAS  PubMed  Google Scholar 

  • Mclaughlin, E. E. &Humphries jr, A. A. (1978) The jelly envelopes and fertilization of eggs of the newt,Notophtalmus viridescens.J. Morph. 158, 73–90.

    Article  CAS  PubMed  Google Scholar 

  • Moreno, A. R. (1972) Histomorfologia del oviducto deBufo arenarum Hensel.Rev. Agronmoica del Noroeste Argentino 9, 585–602.

    Google Scholar 

  • Picheral, B. (1977) La fecondation chez le triton Pleurodéle I. La traversée des enveloppes de l'oeuf par les spermatozoides.J. Ultrastruct. Res. 60, 106–20.

    Article  CAS  PubMed  Google Scholar 

  • Shivers, C. A. &James, J. M. (1971) Morphology and histochemistry of the oviduct and egg-jelly layers in the frogRana pipiens.Anat. Rec. 166, 541–56.

    Article  Google Scholar 

  • Vitaioli, L., Baldoni, E., Fioretti, E., Dubois, G. &Bolognani, L. (1985) Fluctuations in arylsulphatase activity in the rabbit endometrium during the sexual cycle.Histochemistry 83, 127–31.

    Article  CAS  PubMed  Google Scholar 

  • Vitaioli, L., Baldoni, E. &Bellini, L. (1986) Arylsulphatase activity in the frog oviduct during the recovery phase of the reproductive cycle.Cell. Mol. Biol. 32, 187–93.

    CAS  PubMed  Google Scholar 

  • Vitaioli, L., Baldoni, E., Bellini, L., Pederzoli, A. &Bolognani, L., (1987a) Arylsulphatase activity and cerebroside sulphates in the frog oviduct during the reproductive cycle.Histochem. J. 19, 85–93.

    Article  CAS  PubMed  Google Scholar 

  • Vitaioli, L., Baldoni, E. &Bellini, L. (1987b) Arylsulphatase activity in the oviduct of the frogRana esculenta. I. Oestradiol-induced changes following ovariectomy and hypophysectomy.Histochem. J. 19, 217–24.

    Article  CAS  PubMed  Google Scholar 

  • Vitaioli, L., Baldoni, E., Bellini, L., Pederzoli, A. &Bolognani, L. (1988a) Sulphatides in the frog oviduct: response to estradiol stimulation.Cell. Mol. Biol. 34, 373–82.

    CAS  PubMed  Google Scholar 

  • Vitaioli, L., Baldoni, E., Gobbetti, A. &Bellini, L. (1988b) Arylsulphatase activity in the oviduct of the frogRana esculenta. II. Progesterone-induced changes following ovariectomy and hypophysectomy.Histochem. J. 20, 301–7.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Vitaioli, L., Baldoni, E., Bellini, L. et al. Changes induced by progesterone treatment in the sulphatides of the frog oviduct. Histochem J 22, 192–196 (1990). https://doi.org/10.1007/BF02386004

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02386004

Keywords

Navigation