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Electrospray Mass Spectrometry for Assay of Erythromycin A Extracted From Fermentation Liquor

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Biotechnology Techniques

Abstract

Electrospray Mass Spectrometry (ESI-MS) can accurately distinguish and quantify erythromycins by their mass:charge ratios. This procedure is suitable for assay in complex fermentation media, and is compared with previous techniques which depend for detection of erythromycin by the low UV absorbance of erythromycin, after separation of the 5 analogues by HPLC, TLC, GLC, and with biological assays which employ erythromycin-sensitive bacterial strains e.g. of Arthrobacter citreus.

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Authors and Affiliations

  1. Microbiology Group (PABIO), University of Wales Cardiff, PO Box 915 Cardiff, CF1 3TL, Wales, UK

    Janine Morgan & David Lloyd

  2. MDS Panlabs, Sophia House, 28 Cathedral Road, Cardiff, CF1 9LJ, Wales, UK

    Robert T. Rowlands

  3. Welsh School of Pharmacy, University of Wales Cardiff, Redwood Building, King Edward 7th Avenue, Cardiff, CF1 3XF, Wales, UK

    Miranda E. Joyce-Meneksçe & Ian H. Gilbert

Authors
  1. Janine Morgan
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  2. Robert T. Rowlands
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  3. Miranda E. Joyce-Meneksçe
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  4. Ian H. Gilbert
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  5. David Lloyd
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Morgan, J., Rowlands, R.T., Joyce-Meneksçe, M.E. et al. Electrospray Mass Spectrometry for Assay of Erythromycin A Extracted From Fermentation Liquor. Biotechnology Techniques 12, 435–438 (1998). https://doi.org/10.1023/A:1008899020334

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  • DOI: https://doi.org/10.1023/A:1008899020334

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