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Immobilization of β-glucuronidases on an epoxy-activated polyacrylic matrix

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Biotechnology Techniques

Summary

β-Glucuronidases from Escherichia coli, bovine liver and Patella vulgata have been immobilized on an epoxy-activated matrix beads. Optimum binding conditions for pH, NaCl, reaction time and temperature, and support enzymes loading are given, and the immobilized derivatives characterized with respect to pH, temperature, ionic strength, Km and storage stability.

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References

  • Boppana, V.K., Fong, K.L., Ziemniak, J.A. and Lynn, R.K. (1966). J. Chromatogr., 353, 231–247.

    Google Scholar 

  • Bowers, L. and Johnson, P. (1981). Biochim. Biophys Acta, 661, 100–105.

    Google Scholar 

  • Canales, I., Manjōn, A. and Iborra, J.L. (1987) Proc. 4th Europ. Congress Biotechnol., 2, 56–59. Elsevier. Amsterdam.

    Google Scholar 

  • Clifford, C. and Jhonson, D.B. (1980) Biotechnol. Bioeng., 22, 2441–2442.

    Google Scholar 

  • Combie, J.D., Blake, J.W., Nugent, T.E. and Tobin, T. (1981). Clin. Chem., 28, 83–86.

    Google Scholar 

  • Fiddler, M.B. and Gray, G.R. (1978). Anal. Biochem., 86, 716–724.

    Google Scholar 

  • Fink, D.J., Bean, M.K. and Falb, R.D. (1975). Technical report AD-A016 963 for “Army medical research and development command”

  • Hannibal-Friedrich, O., Chun, M. and Sernetz, M. (1980). Biotechnol. Bioeng., 22, 157–175.

    Google Scholar 

  • Hartree, E.I. (1972). Anal. Biochem., 48, 422–427.

    Google Scholar 

  • Iino, N., Yoshidomi, M. and Yoshida, K. (1984), Chem. Phar. Bull., 32, 3710–3714.

    Google Scholar 

  • Kramer, D.M., Lehmann, K., Pennewiss, H. and Plainer, H. (1979). XXVI International IUPAC-Symposium on Macromolecules, Mainz 1979.

  • Levy, G.A., Hay, A.J. and Marsh, C.A. (1957). Biochem. J., 65, 203–208.

    Google Scholar 

  • Marsh, C.A. and Levvy, G.A. (1958). Biochem J., 68, 610–617.

    Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Faculty of Chemistry, University of Murcia, E-30001, Murcia, Spain

    I. Canales, A. Manjōn & J. L. Iborra

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  1. I. Canales
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  2. A. Manjōn
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  3. J. L. Iborra
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Canales, I., Manjōn, A. & Iborra, J.L. Immobilization of β-glucuronidases on an epoxy-activated polyacrylic matrix. Biotechnol Tech 4, 205–210 (1990). https://doi.org/10.1007/BF00222507

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  • DOI: https://doi.org/10.1007/BF00222507

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