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2,3,5-triphenyltetrazolium chloride as a viability assay for immobilized plant cells

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Biotechnology Techniques

Abstract

Grape (V. vinifera L. cv Gamay Fréaux) cells were entrapped in calcium alginate beads. The effect of bead diameter and gel concentration on the viability of the cells was checked. The reduction of 2,3,5-triphenyltetrazolium chloride as well as O2 consumption were used as viability test, and their results compared. The existence of diffusional limitations for O2 introduces an unaccuracy as high as 25% for the O2 consumption method. The reduction assay is more simple, precise and reliable.

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Authors and Affiliations

  1. Dept. of Biochemistry and Molecular Biology B and Immunology, Faculty of Chemistry, University of Murcia, E-30001, Murcia, Spain

    J. L. Iborra, J. Guardiola, S. Montaner, M. Cánovas & A. Manjón

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  1. J. L. Iborra
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  2. J. Guardiola
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  3. S. Montaner
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  4. M. Cánovas
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  5. A. Manjón
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Iborra, J.L., Guardiola, J., Montaner, S. et al. 2,3,5-triphenyltetrazolium chloride as a viability assay for immobilized plant cells. Biotechnol Tech 6, 319–322 (1992). https://doi.org/10.1007/BF02439319

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  • DOI: https://doi.org/10.1007/BF02439319

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