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Transformation of Bacillus brevis 47 by electroporation

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Biotechnology Techniques

Summary

Bacillus brevis 47 was successfully and reproducibly transformed with pUB110 plasmid DNA by electroporation with an efficiency of 104 transformants per μg of DNA. This represents a 10-fold improvement over the chemical transformation method previously used for this organism.

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References

  • Birboim, H.C. and Doly, J., (1979). Nucl. Acids Res. 7, 1513–1523.

    Google Scholar 

  • Dekker, K., Yamagata, H., Sakaguchi, K. and Udaka, S., (1991). Agric. Biol. Chem. 55, 2993–2998.

    Google Scholar 

  • Dower, W.J., Miller, J.F. and Ragsdale, C.W., (1988). Nucl. Acids Res. 16, 6127–6146.

    Google Scholar 

  • Ebisu, S., Takagis, H., Kadowaki, K., Yamagata, H. and Udaka, S., (1992). Biosci. Biotech. Biochem. 56, 812–813.

    Google Scholar 

  • McKenzie, T., Hoshino, T., Tanaka, T. and Sueoka, N., (1986). Plasmid 15, 93–103.

    Google Scholar 

  • Takagi, H., Kagiyama, S., Kadowaki, K., Tsukagoshi, N. and Udaka, S. (1989). Agric. Biol. Chem. 53, 3099–3100.

    Google Scholar 

  • Takahashi, W., Yamagata, H., Yamaguchi, K., Tsukagoshi, N. and Udaka, S. (1983) J. Bacteriol. 156, 1130–1134.

    Google Scholar 

  • Udaka, S. 1976. Agric. Biol. Chem. 40, 523–528.

    Google Scholar 

  • Wight, C.P., Whitney, G.K., White, B.N. and Daugulis, A.J. (1992). Biotechnol. Bioeng. 40, 46–52.

    Google Scholar 

  • Yamada, H., Norihiro, N. and Udaka, S. (1981). J. Bacteriol. 148, 322–332.

    Google Scholar 

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Authors and Affiliations

  1. Department of Chemical Engineering, Queen's University, K7L 3N6, Kingston, Ontario, Canada

    Gordon K. Whitney & Andrew J. Daugulis

  2. Biology Department, McMaster University, L8S 4K1, Hamilton, Ontario, Canada

    Bradley N. White

Authors
  1. Gordon K. Whitney
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  2. Andrew J. Daugulis
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  3. Bradley N. White
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Whitney, G.K., Daugulis, A.J. & White, B.N. Transformation of Bacillus brevis 47 by electroporation. Biotechnol Tech 7, 47–50 (1993). https://doi.org/10.1007/BF00151089

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  • DOI: https://doi.org/10.1007/BF00151089

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