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Release of chloramphenicol acetyl transferase from recombinant Escherichia coli by sonication and the French press

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Biotechnology Techniques

Summary

The release of chloramphenicol acetyl transferase (CAT) from a recombinant Escherichia coli strain by ultrasonication and the French press was compared. French pressing disrupted all cells in suspension whereas only a fraction of the cells was disrupted following sonication. The level of CAT released was highest when cells were totally disrupted. Additional treatment with the detergent Triton X-100 was necessary to maximize CAT recovery, presumably due to association of CAT with cellular debris.

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Authors and Affiliations

  1. Cooperative Research Centre for Tissue Growth and Repair, Department of Chemical Engineering, University of Adelaide, 5005, Adelaide, SA, Australia

    Lene Jorgensen, Brian K. O'Neill & Anton P. J. Middelberg

  2. Department of Microbiology and Immunology, University of Adelaide, 5005, Adelaide, SA, Australia

    Lene Jorgensen, Connor J. Thomas & Renato Morona

Authors
  1. Lene Jorgensen
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  2. Brian K. O'Neill
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  3. Connor J. Thomas
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  4. Renato Morona
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  5. Anton P. J. Middelberg
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Jorgensen, L., O'Neill, B.K., Thomas, C.J. et al. Release of chloramphenicol acetyl transferase from recombinant Escherichia coli by sonication and the French press. Biotechnol Tech 9, 477–480 (1995). https://doi.org/10.1007/BF00159561

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  • DOI: https://doi.org/10.1007/BF00159561

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