Synopsis
Critical thermal maxima (CTM) and genetic variation were compared for red shiners, Notropis lutrensis, from regulated and unregulated sites on the Brazos River in northcentral Texas. Tailwater fish acclimated to 25°C had significantly lower CTM's than those from a site upstream from the dam and unregulated downstream sites. Significantly different intrasite variances were observed, with two- and four-fold larger CTM variances in fish from within 1 km and 30 km of the dam. Genetic variation was determined from electrophoretic comparisons at 21 structural gene loci. Mean heterozygosity was greatest at regulated sites. Tests for locus heterogeneity at five variable loci indicated that regulated and unregulated populations are not homogeneous. Fish under regulation were genetically more similar to each other than they were to those not affected by regulation. The proportions of the gene variance attributable to habitat alteration were partitioned, and fully one-third of the gene variation was attributed to stream regulation. Patterns of variation in thermal tolerance and metabolic enzymes in the red shiner correlated closely with temperature regimes associated with hypolimnion release from the dam. These adaptive responses have occurred in less than 40 years.
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Allendorf, F.W., N. Mitchell, N. Ryman & G. Stahl. 1977. Isoenzyme loci in brown trout (Salmo trutta L.): detection and interpretation from population data. Hereditas 86: 179–190.
Anderson, K.A., T.L. Beitinger & E.G. Zimmerman. 1983. Forage fish assemblages in the Brazos River upstream and downstream from Possum Kingdom Reservoir, Texas, J. Freshw. Ecol. 2: 81–88.
Ayala, F.J., M.L. Tracey, L.G. Barr & J.G. Ehrenfeld. 1974. Genetic and reproductive differentiation of the subspecies, Drosophila equinoxialis caribbensis. Evolution 28: 24–42.
Beitinger, T.L. & L.C. Fitzpatrick. 1979. Physiological and ecological correlates of preferred temperature in fish. Amer. Zool. 19: 319–329.
Brett, J.R. 1971. Energetic responses of salmon to temperature: a study of some thermal relations in the physiology and freshwater ecology of sockeye salmon (Oncorhynchus nerka). Amer. Zool. 11: 99–113.
Brown, J.H. 1971. The desert pupfish. Sci. Amer. 225: 104–110.
Calhoun, S.W. 1981. The role of genic, behavioral and biochemical mechanisms in the adaptation of minnows of the genus Notropis (Cyprinidae) to temperature. M.S. Thesis, North Texas State University, Denton. 71 pp.
Calhoun, S.W., E.G. Zimmerman & T.L. Beitinger. 1982. Stream regulation alters acute temperature preferenda in red shiners, Notropis lutrensis. Can. J. Fish. Aquat. Sci. 39: 360–363.
Chesser, R.K. 1981. Genetic and morphologic variation within and among population of the black-tailed prairie dog. Ph.D. dissertation, University of Oklahoma, Norman. 90 pp.
Dobzhansky, T. 1951. Genetics and the origin of species. Columbia University Press, New York. 446 pp.
Edwards, R.J. 1978. The effect of hypolimnion reservoir releases on fish distribution and species diversity. Trans. Amer. Fish. Soc. 107: 71–77.
Hazel, J.R. & C.L. Prosser. 1974. Molecular mechanisms of temperature compensation in poikilotherms. Phys. Rev. 54: 620–677.
Hedrick, P.W., M.E. Ginevan & E.P. Ewing. 1976. Genetic polymorphism in heterogeneous environments. Ann. Rev. Ecol. Syst. 7: 1–32.
Hochachka, P.W. & G.N. Somero. 1973. Strategies of biochemical adaptation. W.B. Saunders, Philadelphia. 358 pp.
Hutchison, V.H. 1980. The concept of critical thermal maximum. Amer. J. Phys. 237: R367–R368.
Koehn, R.K. 1970. Functional and evolutionary dynamics of polymorphic esterases in catostomid fishes. Trans. Amer. Fish. Soc. 99: 219–228.
Kruskat, W.H. & W.A. Wallis. 1952. Use of ranks in one-way analysis of variance. J. Amer. Statis. Assoc. 47: 583–621.
Levins, R. 1968. Evolution in changing environments. Princeton University Press, Princeton. 120 pp.
Matthews, W.J. & L.G. Hill. 1977. Tolerance of the red shiner, Notropis lutrensis (Cyprinidae), to environmental parameters. S. W. Nat. 22: 89–98.
Matthews, W.J. & L.G. Hill. 1979. Critical thermal maxima, oxygen tolerances and success of cyprinid fishes in a southwestern river. Amer. Midl. Nat. 102: 374–377.
Miller, R.J. & H.W. Robison. 1973. The fishes of Oklahoma. Okla. St. Univ. Press, Stillwater. 246 pp.
Mitton, J.B. & R.K. Koehn. 1975. Genetic organization and adaptive response of allozymes for ecological variables in Fundulus heteroclitus. Genetics 79: 97–111.
Nei, M. 1977. F-statistics and analysis of gene diversity in subdivided populations. Ann. Hum. Genet., Lond. 41: 225–233.
Nei, M. 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89: 583–590.
Otto, R.G. 1973. Temperature tolerance of the mosquitofish, Gambusia affinis. J. Fish Biol. 5: 575–585.
Pfitzer, D.W. 1963. Investigations of waters below large storage reservoirs in Tennessee. Dingel-Johnson Rep., Proj. FIR, Tennessee Fish-Game Comm., Knoxville. 5 pp.
Powell, J.R. 1971. Genetic polymorphisms in varied environments. Science 174: 1035–1036.
Powers, D.A. 1980. Molecular ecology of teleost fish hemoglobins: strategies for adapting to changing environments. Amer. Zool. 20: 139–162.
Powers, D.A. & A.R. Place. 1978. Biochemical genetics of Fundulus heteroclitus (L.) I. Temporal and spatial variation in gene frequencies of LDH-B, MDH-A, GPI-B, and PGM-A. Biochem. Genet. 16: 593–607.
Powers, D.A. & D. Powers. 1975. Predicting gene frequencies in natural population: a testable hypothesis. pp. 63–84. In: Market, C. (ed.) The Isozymes, IV, Academic Press, San Francisco.
Prosser, C.L. 1973. Comparative animal physiology, Vol. 1. W.B. Saunders, Philadelphia. 456 pp.
Ryman, N., C. Reuterwall, K. Nygren & T. Nygren. 1980. Genetic variation and differentiation in Scandinavian moose (Alces alces): are large mammals monomorphic? Evolution 34: 1037–1049.
Selander, R.K., M.H. Smith, S.Y. Yang, W.E. Johnson & J.B. Gentry. 1971. Biochemical polymorphism and systematics in the genus Peromyscus. I. Variation in the oldfield mouse (Peromyscus polionotus). Stud. Genet. IV Univ. Texas Publ. 7103: 49–90.
Shaklee, J.B., J.A. Christiansen, B.D. Slidell, C.L. Prosser & G.S. Whitt. 1977. Molecular aspects of temperature acclimation in fish: contributions of changes in enzyme activities and isozyme patterns to metabolic reorganization in the green sunfish. J. Exp. Zool. 201: 1–20.
Smith, M.W., M.H. Smith & R.K. Chesser. 1983. Biochemical genetics of mosquitofish. I. Environment correlates, and temporal and spatial heterogeneity of allele frequencies within a river drainage. Copeia 1983: 182–193.
Sneath, P.H. & R.R. Sokal. 1973. Numerical taxonomy. W.H. Freeman, San Francisco. 245 pp.
Somero, G.N. 1969. Enzymic mechanisms of temperature compensation: Immediate and evolutionary effects of temperature on enzymes of aquatic poikilotherms. Amer. Nat. 103: 517–530.
Spence, J.A. & H.B.N. Hynes. 1971. Differences in fish populations upstream and downstream of a mainstream impoundment. J. Fish. Res. Board Can. 28: 45–46.
Standford, J.A. & J.V. Ward. 1979. Stream regulation in North America. pp. 215–326. In: J.V. Ward & J.A. Stanford (ed.) The Ecology of Regulated Streams, Proc. 1st. Int. Symp. Reg. Streams, Plenum Press, New York.
Trautman, M.B. & D.K. Gartman. 1974. Re-evaluation of the effects of manmade modifications on Gordon Creek between 1887 and 1973 and especially as regards its fish fauna. Ohio J. Sci. 74: 162–173.
Ushakov, B.P. 1967. Coupled evolutionary changes in protein thermostability. pp. 107–109. In: C.L. Prosser (ed.) Molecular Mechanisms of Temperature Adaptation, American Association for the Advancement of Science, Washington, D.C.
Wright, S. 1965. The interpretation of population structure by F-statistics with special regard to systems of mating. Evolution 9: 395–420.
Zimmerman, E.G. & M.C. Richmond. 1981. Increased heterozygosity at the MDH-B locus in fish inhabiting a rapidly fluctuating thermal environment. Trans. Amer. Fish. Soc. 110: 410–416.
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King, T.L., Zimmerman, E.G. & Beitinger, T.L. Concordant variation in thermal tolerance and allozymes of the red shiner, Notropis lutrensis, inhabiting tailwater sections of the Brazos River, Texas. Environ Biol Fish 13, 49–57 (1985). https://doi.org/10.1007/BF00004855
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DOI: https://doi.org/10.1007/BF00004855