Abstract
Fusarium oxysporum f.sp. lilii (Fol), the causal agent of lily basal rot, produced fusaric acid (FA) in aseptic culture. This toxin induced phytotoxicity symptoms on in vitro-grown lily bulblets of two different cultivars: the Fol-susceptible cultivar was more sensitive to the toxin than the Fol-resistant cultivar. When cultured in the presence of FA, the Fol-susceptible cultivar showed a greater tendency to accumulate FA within its tissues than the Fol-resistant cultivar. The polyphenol oxidase activity of the bulblets was inhibited by 1 mmol L−1 FA in both the cultivars, while at lower FA concentrations the enzyme activity increased only in the Fol-susceptible cultivar. Peroxidase showed a steady activity at the 1 mmol L−1 FA concentration in both the cultivars, while at lower FA dosages its activity increased. Within the Fol-infected in vivo tissues of both the lily cultivars, FA was detectable only in traces. The role of this toxin in the lily basal rot disease seems therefore to be of marginal importance.
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References
Bradford MM (1976) Rapid and sensitive method for the quantitation of microgram quantities of protein utilising the principle of protein-dye binding. Anal Biochem 72: 248-254
Davis D (1969) Fusaric acid in selective pathogenicity of Fusarium oxysporum. Phytopathology 59: 1391-1395
Diez JJ and Gil L (1998) Variability in phenylalanine ammonialyase activity among cell cultures of elm clones inoculated with Ophiostoma novo-ulmi spores. Plant Pathol 47: 687-692
Dobson TA, Desaty D, Brewer D and Vining LC (1967) Biosynthesis of fusaric acid in cultures of Fusarium oxysporum Schlecht. Can J Biochem 45: 809-823
Drysdale RB (1982) The production and significance in phytopathology of toxins produced by species of Fusarium. In: MO Mosse and JE Smith (eds) The Applied Mycology of Fusarium (pp 95-105) Academic Press, New York
Flurkey WH (1985) In vitro biosynthesis of Vicia faba polyphenol oxidase. Plant Physiol 79: 564-567
Grisebach H (1981) Lignins. In: Stumpf PK and Conn EE (eds) The Biochemistry of Plants, Vol 7 (pp 457-478) Academic Press, New York
Heitefuss R, Stahmann MA and Walker JC (1960) Production of pectolytic enzymes and fusaric acid by Fusarium oxysporum f.sp. conglutinans in relation to cabbage yellows. Phytopathology 50: 367-370
Imle EP (1942) Bulb rot disease of lilies. American LilyYearbook 1942 (pp 30-41)
Jullien M (1988) Effects of the Fusarium sp. toxins and selection of crude toxin resistant strains in mesophyll cell cultures of Asparagus officinalis. Plant Physiol Biochem 26: 123-127
Kalyanasundaram R and Venkata Ram CS (1956) Production and systemic translocation of fusaric acid in Fusarium infected cotton plants. J Ind Bot Soc 35: 7-10
Kern H (1972) Phytotoxins produced by Fusaria. In:Wood RKS, Ballio A and Graniti A (eds) Phytotoxins in Plant Disease (pp 35-48) Academic Press, London
Klüpfel D (1957) Uber die Biosynthese und die Umwandlungen der Fusarinsaure in Tomatenpflanzen. Phytopathol Z 29: 349-379
Kuo MS and Scheffer RP (1964) Evaluation of fusaric acid as a factor in the development of Fusarium wilt. Phytopathology 54: 1041-1044
Linderman RG(1981) Fusarium diseases of flowering bulb crops. In: Nelson PE, Toussoun TA and Cook RJ (eds) Fusarium: Diseases, Biology and Taxonomy (pp 129-141) The Pennsylvania State University Press, University Park and London
Löffler HJM and Mouris JR (1992) Fusaric acid: phytotoxicity and in vitro production by Fusarium oxysporum f.sp. lilii, the causal agent of basal rot in lilies. Neth J Pl Pathol 98: 107-115
Löffler HJM, Straathof ThP, Mouris JR and Baayen RP (1995) Durability of resistance in lily to basal rot: evaluation of virulence and aggressiveness among isolates of Fusarium oxysporum f.sp. lilii. European J Pl Pathol 101: 261-271
Murashige T and Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473-497
Nishimura S (1957) Pathological studies on water-melon wilt. On the metabolic products of Fusarium oxysporum f.sp. niveum. Ann Phytopathol Soc Japan 22: 215-219
Remotti PC, Harmelen MJ Van and L¨offler HJM (1997a) In vitro selection for Fusarium-resistance in Gladiolus. Acta Hortic 430: 605-610
Remotti PC, Löffler HJM and Vloten-Doting L Van (1997b) Selection of cell-lines and regeneration of plants resistant to fusaric acid from Gladiolous x grandiflorus cv Peter Pears. Euphytica 96: 237-245
Rudolph K (1976) Non-specific toxins. In: Heitefuss R and Williams PH (eds) Physiological Plant Pathology (p 295) Springer-Verlag, Berlin
Venkata Ram CS (1958) Production of ultraviolet induced mutation in Fusarium vasinfectum with special reference to fusaric acid synthesis. Proc Nat Inst Sci India 23: 117-122
Yoder OC (1980) Toxins in pathogenesis. Annu Rev Phytopathol 18: 103-129
Zieslin N and Ben-Zaken R (1991) Peroxidases, phenylalanine ammonia-lyase and lignification in peduncles of rose flowers. Plant Physiol Biochem 29: 147-151
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Curir, P., Guglieri, L., Dolci, M. et al. Fusaric Acid Production by Fusarium Oxysporum f.sp. lilii and its Role in the Lily Basal Rot Disease. European Journal of Plant Pathology 106, 849–856 (2000). https://doi.org/10.1023/A:1008739708931
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DOI: https://doi.org/10.1023/A:1008739708931