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Effect of culture medium on spore production and germination of races of Colletotrichum lindemuthianum

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Abstract

Spore production on the media neopeptone glucose agar (PGA), malt extract agar (MEA), Czapek Dox agar (CDA) and bean pod extract agar (BEA) was determined for 10 races ofColletotrichum lindemuthianum, as well as the aftereffects on spore germination on water agar.

Clear race-medium interactions were found for spore production. PGA was a universal medium for races of this fungus, but five races gave a much higher production on MEA. CDA and BEA were less effective for spore production. There was no clear aftereffect of the four media on spore germination. The 10 races could be divided into good, moderate and bad germinators, independent of the medium on which they had been developed.

Samenvatting

Onderzoek in vitro werd gedaan naar de invloed van vier cultuurmedia op de sporenproduktie van 10 fysio's vanC. lindemuthianum. Bovendien werd nagegaan of de kieming van de sporen op water-agar afhankelijk was van het medium waarop de sporen waren gevormd.

Er bleken voor sporenproduktie inderdaad aanwijsbare fysio/medium interacties te zijn. De beste algemene gechiktheid had neopepton-glucose-agar (PGA). Op moutextract-agar (MEA) werden echter met verschillende fysio's veel hogere sporenprodukties verkregen, terwijl de sporenproduktie van andere fysio's duidelijk lager lag dan op PGA. De resultaten met Czapek-Dox-agar (CDA) en bonepeul-extract-agar (BEA) waren minder goed. Voor de 10 fysio's kan derhalve met PGA en MEA worden volstaan. Media met natuurlijke extracten (mout, bonepeulen) of met pepton, waren beter voor sporenproduktie dan het synthetische medium Czapek-Dox-agar.

De media hadden geen na-effect op de sporenkieming op water-agar. De fysio's konden echter worden ingedeeld in goede, matige en slechte kiemers.

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References

  • Landes, M. & Hoffmann, G.M., 1979. Zum Keimungs- und Infektionsverlauf beiColletotrichum lindemuthianum aufPhaseolus vulgaris. Phytopathologische Zeitschrift 95: 259–273.

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  • Mathur, R.S., Barnett, H.L. & Lilly, V.G., 1950. Sporulation ofColletotrichum lindemuthianum in culture. Phytopathology 40: 104–114.

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Drijfhout, E., Jansen, J. Effect of culture medium on spore production and germination of races of Colletotrichum lindemuthianum. Netherlands Journal of Plant Pathology 95, 119–122 (1989). https://doi.org/10.1007/BF01997479

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  • DOI: https://doi.org/10.1007/BF01997479

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