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Application of a set of 14 c-DNA probes from wheat to detect restriction fragment length polymorphism (RFLP) in barley

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Summary

A set of 14 probes from wheat cDNA clones was used to search for restriction fragment length polymorphism (RFLP) in six barley lines. The degree of polymorphism among the lines varied greatly between probes and between the various restriction enzymes. Two probes revealed a high degree of polymorphism in all probe/enzyme combinations. Seven of 14 probes did not reveal RFLP. The average level of polymorphism based on all 840 pairwise comparisons was 14.0%, which is higher than has been reported in wheat, but lower than in maize, rice, potato and lettuce. Most of the probes that detected RFLP correspond to sites on the long arms of wheat chromosomes.

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Authors and Affiliations

  1. Department of Plant Breeding (IvP), Agricultural University, P.O. Box 386, 6700 AJ, Wageningen, The Netherlands

    R. E. Niks

  2. Biotechnology Center, Huazhong Agricultural University, 430070, Wuhan, Hubei, Peoples Republic of China

    Y. C. Liao

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  1. Y. C. Liao
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  2. R. E. Niks
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Liao, Y.C., Niks, R.E. Application of a set of 14 c-DNA probes from wheat to detect restriction fragment length polymorphism (RFLP) in barley. Euphytica 53, 115–119 (1991). https://doi.org/10.1007/BF00023791

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  • DOI: https://doi.org/10.1007/BF00023791

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