Summary
Doubled haploid peppers resistant in France to specific strains of tobacco etch virus (TEV) and pepper mottle virus (PeMV) were indexed for resistance to USA strains of TEV and PeMV. The doubled haploids were inoculated when four to six true leaves had developed. The peppers were indexed after an incubation period, and a disease index was calculated. Plants without viral symptoms were reinoculated. Plants challenged to TEV were inoculated four times. Doubled haploids, TEV-resistant in France, were susceptible to the USA strain by the fourth screening. All doubled haploid lines showed TEV symptoms after the fourth inoculation. Two doubled haploids lines, no. 4 and no. 8, had about 6% resistance to TEV after the fourth inoculation. Doubled haploid line no. 5 was 100% resistant to PeMV. The results confirm that the USA PeMV and the USA TEV isolates are different pathotypes than the French ones used originally to screen the doubled haploids. In addition, the use of doubled haploids to identify pathotypes of PeMV and TEV is demonstrated.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Abdalla O.A. & P.R. Desjardins, 1985. Comparative reaction of resistant and susceptible pepper cultivars to California isolates of potyviruses. Phytopathology 75: 1349 (Abstr.).
Abdalla O.A., P.R. Desjardins & J.A. Dodds, 1985. Survey of pepper viruses in California by the ELISA technique. Phytopathology 75: 1311 (Abstr.).
Barrios E.P., H.I. Mosokar & L.L. Black, 1971. Inheritance of resistance to tobacco etch and cucumber mosaic viruses in Capsicum frutescens. Phytopathology 61: 1318.
Benner C.P., C.W. Kuhn, J.W. Demski, J.W. Dobson, P. Colditz & F.W. NutterJr., 1985. Identification and incidence of pepper viruses in northeastern Georgia. Plant Dis. 69: 999–1001.
Bosland P.W., 1992. A comprehensive bibliography of Capsicum. The Chile Institute, Las Cruces, NM 327 pp.
Cook A.A., 1984. ‘Florida VR4’ Bell Pepper. HortScience 19 (3): 456.
Cook, A.A., T.A. Zitter & H.Y. Ozaki, 1977. ‘Delray Bell’, a virus resistant pepper for Florida. Fla. Agric. Exp. Stn. Circular no. S-251. 5 pp.
Greenleaf W.H., 1956. Inheritance of resistance to tobacco-etch virus in Capsicum frutescens and Capsicum annuum. Phytopathology 46: 371–375.
Jensen, C.J., 1986. Haploid induction and production in crop plants. p. 231–256. In: W. Horn, C.J. Jensen, W. Odenbach & O. Schieder (Eds). Genetic Manipulation in Plant Breeding. Proceedings of the International Symposium Organized by EU-CARPIA, 8–13 Sept., 1985. West Berlin, Germany.
Matthews R.E.F., 1981. Plant virology. Academic Press, New York. 897 pp.
Muhyi, R.I. & P.W. Bosland, 1989. A survey identifying pepper viruses occurring in southern New Mexico. ASHS 1989 Annu. Mtg., Tulsa, Okla., Prog. and Abstr. p. 93.
Nelson, M.R., R.E. Wheeler & T.A. Zitter, 1982. Pepper mottle virus. CMI/AAB descriptions of plant viruses, no. 253. Issued jointly by the Commonwealth Mycological Institute and the Association of Applied Biologist, Ferry Lane, Kew, Surrey, England.
Pochard E. & R. Dumas de Vaulx, 1971. La monoploidie chez le piment (Capsicum annuum L.). Z. Pflanzenzüchtung 65: 23–46.
Purcifull, D.W. & E. Hiebert, 1982. Tobacco etch virus. CMI/AAB descriptions of plant viruses, no. 258. Issued jointly by the Commonwealth Mycological Institute and the Association of Applied Biologist, Ferry Lane, Kew, Surrey, England.
Prucifull D.E., T.A. Zitter & E. Hiebert, 1975. Morphology, host range, and serological relationships of pepper mottle virus. Phytopathology 65: 559–562.
Rast A., B. The & C.C.M.M. Stijger, 1987. Disinfection of pepper seed infected with different strains of Capsicum mosaic virus by trisodium phosphate and dry heat treatment. Plant Pathol. 36: 583–588.
Sherf A.F. & A.A. MacNab, 1986. Vegetable diseases and their control. A Wiley-Interscience Publication. New York. 728 pp.
Subramanya R., 1982. Single recessive gene relationship between tolerance and resistance to pepper mottle virus in a cross between C. annuum and C. chinense Jacq. Euphytica 31: 461–464.
Villalon B., 1975. Virus diseases of bell peppers in south Texas. Plant Dis. Rep. 59: 858–862.
Walkey D.G.A., 1985. Applied plant virology. John Wiley & Sons, New York. 329 pp.
Wienbaum Z. & G.M. Milbrath, 1976. The isolation of tobacco etch virus from bell peppers and weeds in southern Illinois. Plant Dis. Rep. 60: 469–471.
Zitter T.A., 1973. Further pepper virus identification and distribution studies in Florida. Plant Dis. Rep. 57: 991–994.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Muhyi, R.I., Bosland, P.W. & Pochard, E. Difference between USA and French isolates of tobacco etch virus and pepper mottle virus displayed by doubled haploid line analysis. Euphytica 72, 23–29 (1993). https://doi.org/10.1007/BF00023769
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00023769