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Potential and limitations of isozymes for chromosomal localization of resistance genes against barley mild mosaic virus (BaMMV)

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Summary

To assess the possibilities offered by isozymes to locate resistance genes against barley mild mosaic virus (BaMMV), the isozyme patterns of 19 barley (Hordeum vulgare L.) genotypes carrying genes different from ym4 were determined. Of the 15 isozyme systems tested, only three were polymorphic, namely aconitate hydratase, esterase, phosphogluconate dehydrogenase, providing markers on four of the seven barley chromosomes. Studies of F2 progenies derived from three crosses between resistant genotypes and susceptible varieties failed to reveal linkage between resistance genes and isozymes. Another goal of the experiment was to study the linkage relationships between ym4 and the esterase locus (Est1-Est2-Est4). Our estimates of the recombination rate between these two loci (3.41 and 8.32%) were in the range of those reported between these esterases and one of the resistance genes of the Chinese variety Mokusekko 3.

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Authors and Affiliations

  1. Institute of Agronomy and Plant Breeding I, Justus-Liebig-University, Ludwigstraße 23, D-35390, Giessen, Germany

    M. Erdogan, W. Friedt & F. Ordon

  2. Laboratoire de Génétique et d'Amélioration des Plantes, INRA, Domaine de Brunehaut, F-80200, Estrées-Mons, France

    J. Le Gouis

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  1. J. Le Gouis
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  2. M. Erdogan
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  3. W. Friedt
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  4. F. Ordon
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Le Gouis, J., Erdogan, M., Friedt, W. et al. Potential and limitations of isozymes for chromosomal localization of resistance genes against barley mild mosaic virus (BaMMV). Euphytica 82, 25–30 (1995). https://doi.org/10.1007/BF00028706

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  • DOI: https://doi.org/10.1007/BF00028706

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