Abstract
The presence of a peptide closely related to porcine NPY has been demonstrated in the goldfish brain and pituitary by means of radioimmunoassay (RIA) and high performance liquid chromatography (HPLC). The RIA data demonstrate that displacement curves of brain extracts are parallel to a porcine NPY standard and that in HPLC a compound present in brain extracts is co-eluted with porcine NPY. The distribution of this NPY-like factor within the central nervous system was studied by radioimmunoassay and immunohistochemistry. The results indicated that NPY has a widespread distribution with the highest concentrations being found in the telencephalon and diencephalon. In the pituitary gland, NPY immunoreactive terminals characterized at the electron microscope level were found in the different lobes and, in particular, in close association with the gonadotrophin (GTH) secreting cells. Using anin vitro perifusion system, it was shown that NPY causes a dose dependent increase of GTH release from anterior lobe fragments.
These data indicate for the first time in teleosts that NPY is present and widely distributed in the brain and pituitary, and that among other putative functions, could be implicated in the multihormonal release of GTH from the pituitary.
Similar content being viewed by others
References cited
Allen, Y.S., Adrian, T.E., Allen, J.M., Tatemoto, K., Crow, T.J., Bloom, S.R. and Polak, J.M. 1983. Neuropeptide Y distribution in the rat brain. Science 221: 877–879.
Breton, B., Micolasczik, T., Danger, J.M., Gonnet, F. and Vaudry, H. 1988. Neuropeptide Y stimulatesin vitro gonadotrophin secretion in the rainbow trout. Fish Physiol. Biochem. (In press).
Chronwall, B.M., DiMaggio, D.A., Massari, V.J., Ruggiero, D.A. and O'Donohue, T.L. 1985. The anatomy of Neuropeptide Y-containing neurons in rat brain. Neuroscience 15: 1159–1181.
Ciofi, P., Croix, D. and Tramu, G. 1987. Coexistence of hGHRF and NPY immunoreactivities in neurons of the arcuate nucleus of the rat. Neuroendocrinology 45: 425–428.
Danger, J.M., Guy, J., Benyamina, M., Jégou, S., Leboulenger, F., Coté, J., Tonon, M.C., Pelletier, G. and Vaudry, H. 1985. Localization and identification of Neuropeptide Y (NPY)-like immunoreactivity in the frog brain. Peptides 6: 1225–1236.
Danger, J.M., Leboulenger, F., Guy, J., Tonon, M.C., Benyamina, M., Martel, J.C., Saint-Pierre, S., Pelletier, G. and Vaudry, H. 1986. Neuropeptide Y in the intermediate lobe of the frog pituitary acts as an alpha-MSH-release inhibiting factor. Life Sci. 39: 1183–1192.
Everitt, B.J., Hökfelt, T., Terenius, L., Tatemoto, K., Mutt, V. and Goldstein, M. 1984. Differential co-existence of Neuropeptide Y (NPY)-like immunoreactivity with catecholamines in the central nervous system of the rat. Neuroscience 11: 443–462.
Geffard, M., Patel, S., Dulluc, J. and Rock, A.M. 1986. Specific detection of noradrenaline in the rat brain by using antibodies. Brain Res. 393: 395–400.
Gray, T.S. and Morley, J.E. 1986. Neuropeptide Y: anatomical distribution and possible function in mammalian nervous system. Life Sci. 38: 398–401.
Kah, O., Dubourg, P., Onteniente, B., Geffard, M. and Calas, A. 1984. The dopaminergic innervation of the goldfish pituitary. Cell Tiss. Res. 244: 577–582.
Kalra, S.P. and Crowley, W.R. 1984. Norepinephrine-like effects of neuropeptide Y on LH release in the rat. Life Sci. 35: 1173–1176.
Kerkerian, L., Guy, J., Lefevre, G. and Pelletier, G. 1985. Effects of Neuropeptide Y (NPY) on the release of anterior pituitary hormones in the rat. Peptides 6: 1201–1204.
Kimmel, J.R., Plisetskaya, E.M., Pollock, H.G., Hamilton, J.W., Rouse, J.B., Ebner, K.E. and Rawitch, A.B. 1986. Structure of a peptide from coho salmon endocrine pancreas with homology to neuropeptide Y. Biochem. Biophys. Res. Comm. 141: 1084–1091.
Lundberg, J.M., Terenius, L., Hökfelt, T., Martling, C.R., Tatemoto, K., Mutt, V., Polak, J., Bloom, S. and Goldstein, M. 1982. Neuropeptide Y (NPY)-like immunoreactivity in peripheral noradrenergic neurons and effects of NPY on sympathetic functions. Acta Physiol. Scand. 116: 447–480.
Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265–275.
McDonald, J.K., Lumpkin, M.D., Samson, W.K. and McCann, S.M. 1985. Neuropeptide Y affects secretion of luteinizing hormone and growth hormone in ovariectomized rats. Proc. Nat. Acad. Sci. U.S.A. 82: 561–564.
Osborne, N.N., Patel, S., Terenghi, G., Allen, J.M., Polak, J.M. and Bloom, S.R. 1985. Neuropeptide Y (NPY)-like immunoreactive amacrine cells in retinas of frog and goldfish. Cell Tis. Res. 241: 651–656.
Pelletier, G., Désy, L., Kerkérian, L. and Coté, J. 1984. Immunocytochemical localization of Neuropeptide Y (NPY) in the human hypothalamus. Cell Tiss. Res. 238: 203–205.
Rodriguez-Sierra, J.F., Jacobowitz, D.M. and Blake, C.A. 1987. Effects of neuropeptide Y on LH, FSH and TSH release in male rats. Peptides 8: 539–542.
Tatemoto, K., Carlquist, M. and Mutt, V. 1982. Neuropeptide Y — a novel brain peptide with structural similarities to peptide YY and pancreatic peptide. Nature, Lond. 296: 659–660.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kah, O., Pontet, A., Danger, J.M. et al. Characterization, cerebral distribution and gonadotropin release activity of neuropeptide Y (NPY) in the goldfish. Fish Physiol Biochem 7, 69–76 (1989). https://doi.org/10.1007/BF00004691
Issue Date:
DOI: https://doi.org/10.1007/BF00004691