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Some features about transposition of the maize elementDissociation inNicotiana plumbaginifolia

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Abstract

We have recently shown that a plasmid-borneDissociation (Ds) element can excise from extrachromosomal plasmid DNA and integrate into a plant genome in the presence of theActivator (Ac) transposase.Ds andAc-carrying plasmids were used to co-transformNicotiana plumbaginifolia protoplasts. Transgenic plants were regenerated and analyzed. Here we describe further characterization of the system and discuss its efficiency in terms of DNA transformation and transposon tagging.

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  1. Laboratoire de Biologie Cellulaire, INRA, Route de St Cyr, 78026, Versailles cedex, France

    Nicole Houba-Hérin, Monique Domin & Anne-Sophie Leprince

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  1. Nicole Houba-Hérin
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  2. Monique Domin
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  3. Anne-Sophie Leprince
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Houba-Hérin, N., Domin, M. & Leprince, AS. Some features about transposition of the maize elementDissociation inNicotiana plumbaginifolia . Genetica 93, 41–48 (1994). https://doi.org/10.1007/BF01435238

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  • DOI: https://doi.org/10.1007/BF01435238

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