Abstract
The relationship between chilling tolerance of six rice cultivars – Facagro 57, Facagro 76, Fujisaka 5, Kirundo 3, Kirundo 9 and IR64 -and the fatty acid composition in total lipids, phospholipids, galactolipids and neutral lipids from leaves was studied. Higher double bond index and proportions of linolenic acid in the phospholipid and galactolipid classes were related to cultivar chilling tolerance, but this was not so for the total lipids nor the neutral lipid class. The somaclonal families derived from Facagro 76, Kirundo 3 and Kirundo 9 that showed enhanced chilling tolerance as compared to their original parental cultivar were analyzed for fatty acid composition in phospholipids and galactolipids from leaves. Altered proportions in fatty acid composition in phospholipids, galactolipids or both were found in the somaclonal families derived from Facagro 76 and Kirundo 9, but not from Kirundo 3. These changes most usually resulted in higher double bond index and higher proportions in linoleic and linolenic acids which were related either to lower ratio of C16 to C18 fatty acids or to higher unsaturation in the C18 fatty acid fraction. Different mechanisms thus seem to be implicated in the altered fatty acid composition of somaclones, which may be related to the chilling tolerance improvement of some somaclonal families.
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Adkins SW, Shiraishi T, McComb JA, Ratanopol S, Kupkanchanakul T, Armstrong LJ and Schultz AL (1990) Somaclonal variation in rice – Submergence tolerance and other agronomic characters. Physiol Plant 80: 647–654
Adkins SW, Kunanuvatchaidach R and Godwin ID (1995) Somaclonal variation in rice – Drought tolerance and other agronomic characters. Aust J Bot 43: 201–209
Bertin P, Kinet JM and Bouharmont J (1995) Heritable chilling tolerance improvement in rice through somaclonal variation and cell line selection. Aust J Bot 44: 91–103
Bertin P, Bouharmont J and Kinet JM (1996a) Somaclonal variation and chilling tolerance improvement in rice: changes in chilling-induced electrolyte leakage. Plant Breed 115: 268– 272
Bertin P, Bouharmont J and Kinet JM (1996b) Evaluation of chilling sensitivity in different rice cultivars. Relationship between screening procedures applied during germination and vegetative growth. Euphytica 89: 201–210
Chang TT and Oka HI (1976) Genetic variousness in the climatic adaptation of rice cultivars. Climate and Rice, pp 87–111. Los Baños, Philippines: International Rice Research Institute
Futsuhara Y and Toriyama K (1966) Genetic studies on cool tolerance in rice. III. Linkage relations between genes controlling cold tolerance and marker genes Nagao and Takahasi. Jap J Breed 16: 231–242
Futsuhara Y and Toriyama K (1969) Genetic studies on cool tolerance in rice. IV. Direct and indirect effects of selection and cold tolerance. Jap J Breed 19: 286–292
Futsuhara Y and Toriyama K (1971) Genetic studies on cool tolerance in rice. V. Effectiveness of individual and line selections for cool tolerance. Jap J Breed 21: 181–188
Hugly S and Somerville C (1992) A role for membrane lipid polyunsaturation in chloroplast biogenesis at low temperature. Plant Physiol 99: 197–202
Kaw RN and Khush GS (1986) Combining ability for low temperature tolerance in rice. Rice genetics, pp 593–612. Los Baños, Philippines: International Rice Research Institute
Kodama H, Hamada T, Horiguchi G, Nishimura M and Iba K (1994) Genetic enhancement of cold tolerance by expression of a gene for chloroplast !-3 fatty acid desaturase in transgenic tobacco. Plant Physiol 105: 601–605
Kucherenko LA (1980) Tissue culture in rice improvement: Experiences in the USSR. Innovative Approaches to Rice Breeding, pp 93–102. Los Baños, Philippines: International Rice Research Institute
Kucherenko LA (1991) Rice improvement through tissue culture in the USSR. In: YPS Bajaj (ed.) Biotechnology in Agriculture and Forestry 14, pp. 575–590. Berlin, Heidelberg: Springer-Verlag
Li CC and Rutger JN (1980) Inheritance of cool-temperature seedling vigour in rice and its relationship with other agronomic characters. Crop Sci 20: 295–298
Li T, Lynch DV and Steponkus PL (1987) Molecular species composition of phosphatidylglycerols from rice cultivars differing in chilling sensitivity. Cryo-Lett 8: 314–322
Lyons JM and Raison JK (1970) Oxidative activity of mitochondria isolated from plant tissues sensitive and resistant to chilling. Plant Physiol 45: 386–389
Majumder MK, Seshu DV and Shenoy VV (1989) Implications of fatty acids and seed dormancy in a new screening procedure for cold tolerance in rice. Crop Sci 29: 1298–1304
Murata N (1983) Molecular species composition of phosphatidylglycerols from chilling-sensitive and chilling-resistant plants. Plant Cell Physiol 24: 81–86
Murata N and Nishida I (1990) Lipids in relation to chilling sensitivity of plants. In: CY Wang (ed.) Chilling injury of horticultural crops, pp 181–199. Florida: CRC Press
Murata N, Ishizaki-Nishizawa O, Higashi S, Hayashi H, Tasaka Yand Nishida I (1992) Genetically engineered alteration in the chilling sensitivity of plants. Nature 356: 710–713
Murata N, Sato N, Takahashi N and Hamazaki Y (1982) Compositions and positional distributions of fatty acids in phospholipids from leaves of chilling-sensitive and chilling-resistant plants. Plant Cell Physiol 23: 1071–1079
Raison JK and Orr GR (1990) Proposals for a better understanding of the molecular basis of chilling injury. In: CY Wang (ed.) Chilling injury of horticultural crops, pp 145–164. Florida: CRC Press
Raison JK and Wright LC (1983) Thermal phase transitions in the polar lipids of plant membranes. Their induction by disaturated phospholipids and their possible relation to chilling injury. Biochim Biophys Acta 731: 69–78
St john JB, Christiansen MN, Ashworth EN and Gentner WA (1979) Effect of BASF 13-338, a substituted pyridazinone, on linolenic acid levels and winterhardiness of cereals. Crop Sci 19: 65–69
Sun ZX, Sun LH and Shu LH (1991) Utilization of somaclonal variation in rice breeding. In: YPS Bajaj (ed.) Biotechnology in Agriculture and Forestry 14, pp 329–346. Berlin Heidelberg: Springer-Verlag
Toriyama K and Futsuhara Y (1960) Genetic studies on cool tolerance in rice. Jap J Breed 10: 143–152
Van Sint Jan V (1992) Sélection in vitro et caractérisation de lignées de cellules et de plantes d'Oryza sativa L. tolérantes á l'aluminium. Ph.D. thesis, Université Catholique de Louvain, Belgium
Wolter FP, Schmidt R and Heinz E (1992) Chilling sensitivity of Arabidopsis thalianawith genetically engineered membrane lipids. EMBO J 11: 4685–4692
Wu J and Browse J (1995) Elevated levels of high-meltingpoint phosphatidylglycerols do not induce chilling sensitivity in an Arabidopsis mutant. Plant Cell 7: 17–27
Wu J, James DW Jr, Dooner HK and Browse J (1994) A mutant of Arabidopsis deficient in the elongation of palmitic acid. Plant Physiol 106: 143–150
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Bertin, P., Bullens, P., Bouharmont, J. et al. Somaclonal variation and chilling tolerance improvement in rice: Changes in fatty acid composition. Plant Growth Regulation 24, 31–41 (1998). https://doi.org/10.1023/A:1005950113741
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DOI: https://doi.org/10.1023/A:1005950113741