Skip to main content
SpringerLink
Log in

A modified continuous flow microcalorimeter for measuring heat dissipation by mammalian cells in batch culture

  • Biological/Life Sciences
  • Published:
Journal of thermal analysis Aims and scope Submit manuscript

Abstract

Microcalorimeters to monitor the heat dissipation of bench-scale animal cell cultures on line and in real time require a continuous circuit between the vessel measuring heat flow rate and the bioreactor. The modifications to the transmission lines and calorimetric heat exchanger were to: (i) reverse the usual upward direction of the cell suspension in the flow vessel to downwards; (ii) install an in situ washing/cleaning facility; (iii) use low diffusivity PEEK material; and (iv) maintain thermal equilibration by water-jacketing the transmission tubing. Chemical calibration showed that there was more than a 20% difference between the physical volume and the effective thermal volume. An appropriate thermodynamic system was defined in order to permit enthalpy balance studies.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. U. von Stockar and I. W. Marison, Thermochim. Acta, 193 (1991) 215.

    Article  Google Scholar 

  2. L. Gustafsson, Thermochim. Acta, 193 (1991) 145.

    Article  CAS  Google Scholar 

  3. P. M. Hayter, E. M. A. Curling, A. J. Baines, N. Jenkins, I. Salmon, P. G. Strange and A. T. Bull, Appl. Microbiol. Biotechnol., 34 (1991) 559.

    Article  CAS  Google Scholar 

  4. R. B. Kemp in A. E. Beezer (Ed.), Biological MicrocaIorimetry, Academic Press, London 1980, p. 113.

    Google Scholar 

  5. R. B. Kemp in A. M. James (Ed.), Thermal and Energetic Studies of Cellular Biological Systems, Wright, Bristol 1987, p. 147.

    Google Scholar 

  6. S. Pauly, in J. Brandrup and E. H. Immergut (Eds.), Polymer Handbook, Wiley, London 1989, p. 435.

    Google Scholar 

  7. R. B. Kemp, Methods Mol. Biol., 43 (1995) 211.

    CAS  Google Scholar 

  8. J. Suurkuusk and I. Wadsö, Chem. Scripta., 20 (1982) 155.

    CAS  Google Scholar 

  9. C. Larsson, A. Blomberg and L. Gustafsson, Biotechnol. Bioeng., 38 (1991) 447.

    Article  CAS  Google Scholar 

  10. A. Schön and I. Wadsö, J. Biochem. Biophys. Methods, 13 (1986) 135.

    Article  Google Scholar 

  11. I. Wadsö, in K. N. Marsh and P. A. G. O'Hare (Eds.), Solution Calorimetry, Blackwell Scientific Publications, Oxford 1994, p. 267.

    Google Scholar 

  12. I. Wadsö, Thermochim. Acta, 219 (1993) 1.

    Article  Google Scholar 

  13. A. Chen and I. Wadsö, J. Biochem. Biophys. Methods, 6 (1982) 297.

    Article  CAS  Google Scholar 

  14. E. Gnaiger and R. B. Kemp, Biochim. Biophy. Acta, 1016 (1990) 328.

    Article  CAS  Google Scholar 

  15. U. von Stockar, L. Gustafsson, K. Larsson, I. Marison, P. Tissot and E. Gnaiger, Biochim. Biophys. Acta, 1183 (1993) 221.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Biological Sciences, The University of Wales, Penglais, SY23 3DA, Aberystwyth, Wales, UK

    Y. Guan, P. C. Lloyd, P. M. Evans & R. B. Kemp

Authors
  1. Y. Guan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. C. Lloyd
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. P. M. Evans
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. R. B. Kemp
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

We are indebted to the BBSR.C (UK) for financial support under grant No. 2/3680. Mr. R.L. Taylor (ThermoMetric Ltd, Northwich, Cheshire) has been extremely helpful in discussions about the various designs.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Guan, Y., Lloyd, P.C., Evans, P.M. et al. A modified continuous flow microcalorimeter for measuring heat dissipation by mammalian cells in batch culture. Journal of Thermal Analysis 49, 785–794 (1997). https://doi.org/10.1007/BF01996761

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01996761

Keywords

Search

Navigation