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Microcalorimetric Investigations of K+- and Mg2+-Induced Polymerization of Actin at Temperatures from 293.15 K to 310.15 K

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Abstract

Microcalorimetric measurements of the polymerization of actin in the presence of 100 mM KCl and 2 mM MgCl2were carried out with a Calvet MS-80 microcalorimeter at temperatures from 293.15 to 310.15 K. It was observed that the polymerization of actin was endothermic and the enthalpy change for actin polymerization was temperature-dependent. The enthalpy change ΔHowas fitted to ΔHo(kJ mol-1)=434.0-1.16 (T/K) and the change in heat capacity ΔCp ocalculated from ΔHowas -1.16 kJ (mol K)-1in the above range of temperatures. The direct calorimetry results showed that the enthalpy and entropy change for actin polymerization could not be obtained from measurements of the critical concentration and the only way to assess the enthalpy change for the polymerization of actin and similar reactions lies in the use of calorimetry.

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References

  1. P. Sheterline and J. C. Sparrow, Actin, Protein Profile, 1 (1994) 5.

    Google Scholar 

  2. S. A. Rich and J. E. Estes, J. Mol. Biol., 104 (1976) 777.

    Article  CAS  Google Scholar 

  3. J. A. Cooper and T. D. Pollard, Methods in Enzymology, Academic Press, New York, 85 (part B) (1982) 182.

    Google Scholar 

  4. S. Asakura, M. Kasai and F. Oosawa, J. Polymer Sci., 44 (1960) 35.

    Article  CAS  Google Scholar 

  5. M. Kasai, Biochim. Biophys. Acta, 180 (1969) 399.

    Article  CAS  Google Scholar 

  6. D. J. Gordon, Y. Z. Yang and E. D. Korn, J. Biol. Chem., 251 (1976) 7474.

    CAS  Google Scholar 

  7. L. A. Selden, L. C. Gershman and J. E. Estes, J. Muscle Res. Cell Motil., 7 (1986) 215.

    Article  CAS  Google Scholar 

  8. H. J. Kinosian, L. A. Selden, J. E. Estes and L. C. Bershman, Biochim. Biophys. Acta, 1077 (1991) 151.

    CAS  Google Scholar 

  9. A. Holtzer and M. F. Holtzer, J. Phys. Chem., 78 (1974) 1442.

    Article  CAS  Google Scholar 

  10. J. P. Weir and D. W. Frederiksen, Arch. Biochem. Biophys., 203 (1980) 1.

    Article  CAS  Google Scholar 

  11. H. H. Zeng, B. H. Wang, Y. M. Zhang and K. Wang, Thermochim. Acta, 265 (1995) 31.

    Article  Google Scholar 

  12. H. H. Zeng, K. Wang, B. H. Wang and Y. M. Zhang, Int. J. Biological Macromol., 18 (1996) 161.

    Article  CAS  Google Scholar 

  13. H. H. Zeng, B. H. Wang, Y. M. Zhang and K. Wang, Thermochim. Acta, (1997) for submission.

  14. X. Liu, B. H. Wang, S. Shu, Y. M. Zhang and L. F. Yen, Thermochim. Acta, (1997) in press.

  15. J. D. Pardee and J. A. Spudich, Methods in cell biology, Academic Press, New York 24 (1982) 271.

    Google Scholar 

  16. C. Frieden, Biochemistry, 80 (1983) 6513.

    CAS  Google Scholar 

  17. T. Kodama, Physiol. Rev., 65 (1985) 467.

    CAS  Google Scholar 

  18. S. Higashi and F. Oosawa, J. Mol. Biol., 12 (1965) 843.

    Article  CAS  Google Scholar 

  19. E. D. Korn, Physiol. Rev., 62 (1982) 674.

    Google Scholar 

  20. L. S. Tobacman and E. D. Korn, J. Biol. Chem., 258 (1983) 3207.

    CAS  Google Scholar 

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Authors and Affiliations

  1. Institute of Physical Chemistry, Beijing University, Beijing, 100871

    J. Tian, B. H. Wang, Z. F. Li & Y. M. Zhang

  2. College of Biological Science, Beijing Agricultural University, Beijing, 100094

    S. C. Han

  3. School of Pharmaceutical Science, Beijing Medical University, Beijing, 100083, China

    H. H. Zeng

Authors
  1. J. Tian
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  2. S. C. Han
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  3. H. H. Zeng
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  4. B. H. Wang
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  5. Z. F. Li
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  6. Y. M. Zhang
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Tian, J., Han, S.C., Zeng, H.H. et al. Microcalorimetric Investigations of K+- and Mg2+-Induced Polymerization of Actin at Temperatures from 293.15 K to 310.15 K. Journal of Thermal Analysis and Calorimetry 54, 775–783 (1998). https://doi.org/10.1023/A:1010187719349

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  • DOI: https://doi.org/10.1023/A:1010187719349

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