Abstract
The difficulty during SPPS in acylating thesecondary amino group of Htc, a locally constrainedtyrosine, can be correlated with the steric hindranceof the amino acid or with the conformation of the growingpeptide chain. Our experimental data indicate that theavailability of the Htc amino group is associated with itssteric hindrance rather than a conformational effectof the peptide chain.An optimized solid phase automated protocol for Htcis reported. Under optimal conditions, Fmoc-aminoacids with hindered side chains were incorporated inapproximately 99% yield using HATU as couplingreagent. Unhindered side chain amino acid acylated thesecondary amino group of Htc in good yield underclassical HBTU/HOBt coupling conditions.
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Hruby, V.J., Li, G., Haskett-Luevano, C. and Shenderovich, M., Biopolymers Pept. Sci., 43 (1997) 219.
Ruzza, P., Donella-Deana, A., Calderan, A., Filippi, B., Cesaro, L., Pinna, L.A. and Borin, G., J. Pept. Sci., 2 (1996) 325.
Model 431A Peptide Synthesizer User's Manual, Applied Biosystems, Inc., Foster City, CA, 1989.
Dölling, R., Beyermann, M., Haenel, J., Kernchen, F., Krause, E., Franke, P., Brudel, M. and Bienert, M., In Maia, H.L.S. (Ed.), Peptides 1994, Proceedings of the 23rd European Peptide Symposium, September 4–10, 1994, Braga, Portugal, ESCOM, Leiden, 1995, pp. 244–245.
Kaiser, E., Colescott, R.L., Bossinger, C.D. and Cook, P.I., Anal. Biochem., 34 (1970) 595.
Meienhofer, J., Waki, M., Heimer, E.P., Lambross, T.J., Makofske, R.C. and Chang, C.D., Int. J. Pept. Protein Res., 13 (1979) 35.
Ruzza, P., Calderan, A., Donella-Deana, A., Biondi, B., Ancona, B., Pinna, L.A. and Borin, G., 16th American Peptide Symposium, Program & Abstracts, P673 (1999) 309.
Ötvös, F., Murphy, R.F. and Lovos, S., J. Pept. Res., 53 (1999) 302.
Albericio, F. and Carpino, L.A., Methods Enzymol., 289 (1997) 104.
Tourwé, D., Toth, G., Lebl, M., Verschueren, K., Knapp, R.J., Davis, P., Van Binst, G., Yamamura, H.I., Burks, T.K., Kramer, T. and Hruby, V.J., In Smith, J.A. and Rivier, E. (Eds.), Peptides. Chemistry and Biology, Proceedings of the 12th American Peptide Symposium, ESCOM, Leiden, 1992, pp. 307–308.
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Ruzza, P., Calderan, A., Cavaggion, F. et al. Solid-phase synthesis of an Htc-containingdimer analog of the autophosphorylationsite of pp60src PTK: Effective acylationconditions for Htc residues. Letters in Peptide Science 7, 79–83 (2000). https://doi.org/10.1023/A:1008912426423
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DOI: https://doi.org/10.1023/A:1008912426423