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Replication of alcelaphine herpesviruses in various cell culture systems and subsequent purification of virus

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Journal of tissue culture methods

Summary

Cultures of fetal aoudad sheep kidney (FAK), bovine embryonic lung (BEL), and African green monkey kidney (Vero) cells were compared for differential replication of alcelaphine herpesviruses. Cell-free virus appears more rapidly when infected cells are incubated at 33° C rather than at 37° C. Events in the replication and morphologic development of several alcelaphine herpesvirus isolates have been documented using light and electron microscopy. Techniques for indirect immunofluorescence and serum virus neutralization are described. When virus free of host-cell contaminants is desired for biochemical analysis, virus isolates are initially purified on sucrose gradients and then further purified by density gradient centrifugation in Percoll.

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Authors and Affiliations

  1. Virology and Immunology Laboratory, Center for Reproduction of Endangered Species, Zoological Society of San Diego, 92112, San Diego, California

    Bruce S. Seal, Robert B. Klieforth & Werner P. Heuschele

  2. California Veterinary Diagnostic System, Virology Laboratory, University of California-Davis, 95616, Davis, California

    Anthony E. Castro

Authors
  1. Bruce S. Seal
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  2. Robert B. Klieforth
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  3. Anthony E. Castro
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  4. Werner P. Heuschele
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Seal, B.S., Klieforth, R.B., Castro, A.E. et al. Replication of alcelaphine herpesviruses in various cell culture systems and subsequent purification of virus. Journal of Tissue Culture Methods 11, 49–55 (1988). https://doi.org/10.1007/BF01404132

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  • DOI: https://doi.org/10.1007/BF01404132

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