Summary
Two procedures are described for quantitating fluid-phase endocytosis in monolayer cultures of opossum kidney epithelial cells. Both procedures use the same principle which is to determine uptake of a specific, easily detectable compound in the extracellular fluid. One method uses lucifer yellow, the other uses horseradish peroxidase.
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M. I. A. was supported by a Postdoctoral Fellowship from the American Heart Association, Indiana Affiliate Inc. S. A. K. was supported by a Research Career Development Award and grant DK32148 from the National Institutes of Health.
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Abraham, M.I., Kempson, S.A. Quantitation of fluid-phase endocytosis by proximal tubular cells in culture. Journal of Tissue Culture Methods 13, 191–194 (1991). https://doi.org/10.1007/BF02388125
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DOI: https://doi.org/10.1007/BF02388125