Summary
A procedure is described for the in vitro culture ofXiphophorus embryos. The method permits the raising of embryos of viviparous fish species from an early stage of embryo-genesis (first somite formation) beyond the stage of birth and into fertile adults.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Dulbecco, R.; Vogt, M. Plaque formation and isolation of pure lines with poliomyelitis viruses. J. Exp. Med. 98: 167–168; 1954.
Gordon, M. Fishes as laboratory animals. In: Farris, E., ed. The care and breeding of laboratory animals. New York: John Wiley and Sons; 1950: 345–449.
Gordon, M. The melanoma cell as an incompletely differentiated pigment cell.In: Gordon, M. ed. Pigment cell biology. New York: Academic Press; 1959: 215–239.
Haas-Andela, H.In vitro-Kultur und Aufzucht von Embryonen lebendgebärender Zahnkarpfen der GattungXiphophorus. Zool. Anz. (Jena) 197: 1–5; 1976.
Humm, D. G.; Young, R. S. The embryological origin of pigment cells in platyfish-swordtail hybrids. Zoologica 41: 1–10; 1956.
Kallman, K. D. The platyfish,Xiphorus maculatus. In: King, R. C., ed. Handbook of genetics, vol. 4. New York: Plenum Press; 1975: 81–132.
Scrimshaw, N. S. Embryonic development in poeciliid fishes. Biol. Bull. 88: 233–246; 1945.
Tavolga, W. N. Embryonic development of the platyfish(Platypoecilus maculatus), the swordtail(Xiphophorus helleri), and their hybrids. Bull. Am. Mus. Nat. Hist. 94: 165–229; 1949.
Tavolga, W. N.; Rugh, R. Development of the platyfish,Platypoecilus maculatus. Zoologica 32: 1–15; 1947.
Vielkind, J.; Haas-Andela, H.; Anders, F. DNA-mediated transformation in the platyfish-swordtail melanoma system. Experientia 32: 1043–1044; 1976.
Vielkind, J.; Haas-Andela, H.; Vielkind, U.; Anders, F. The induction of a specific pigment cell type by total genomic DNA injected into the neural crest region of fish embryos of the genusXiphophorus. Mol. Gen. Genet. 185: 379–389; 1982.
Vielkind, J.; Schwab, M.; Anders, F. Fate of bacterial DNA injected into embryos of poeciliid fish. In: Schröder, J. H., ed. Genetics and mutagenesis of fish. Berlin, Heidelberg, New York: Springer Verlag; 1973: 123–137.
Vielkind, J.; Vielkind, U. Melanoma formation in fish of the genusXiphophorus: a genetically-based disorder in the determination and differentiation of a specific pigment cell. Can. J. Genet. Cytol. 24: 133–149; 1982.
Vielkind, U.; Vielkind, J. The culture of embryos and cells from platyfish and swordtails for carcinogenicity testing and studies on melanoma formation. Natl. Cancer Inst. Monogr. 65: 179–183; 1984.
Wourms, J. P. Annual Fishes. Wilt, F. H. and Wessells, N. K. Eds. Methods in developmental biology. New York: Crowell; 1967: 123–137.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Vielkind, U., Vielkind, J. Culture of embryos from viviparous fishes of the genusXiphophorus . Journal of Tissue Culture Methods 8, 73–78 (1983). https://doi.org/10.1007/BF02099242
Issue Date:
DOI: https://doi.org/10.1007/BF02099242