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Extreme resistance to cucumber mosaic virus (CMV) in transgenic tomato expressing one or two viral coat proteins

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Abstract

For the production of broad commercial resistance to cucumber mosaic virus (CMV) infection, tomato plants were transformed with a combination of two coat protein (CP) genes, representing both subgroups of CMV. The CP genes were cloned from the CMV-D strain and Italian CMV isolates (CMV-22 of subgroup I and CMV-PG of subgroup II) which have been shown to produce severe disease symptoms. Four plant transformation vectors were constructed: pMON18774 and pMON18775 (CMV-D CP), pMON18831 (CMV-PG CP) and pMON18833 (CMV-22 CP and CMV-PG CP). Transformed R0 plants were produced and lines were selected based on the combination of three traits: CMV CP expression at the R0 stage, resistance to CMV (subgroup I and/or II) infection in growth chamber tests in R1 expressing plants, and single transgene copy, based on R1 segregation. The results indicate that all four vector constructs generated plants with extremely high resistant to CMV infection. The single and double gene vector construct produced plants with broad resistance against strains of CMV from both subgroups I and II at high frequency. The engineered resistance is of practical value and will be applied for major Italian tomato varieties.

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Authors and Affiliations

  1. Monsanto Co., 700 Chesterfield Parkway, St. Louis, MO, 63198, USA

    Wojciech Kaniewski, T. Mitsky & J. Layton

  2. Istituto Sperimentale per la Patologia Vegetale, Via C. G. Bertero, 22, 00156  , Roma, Italy

    Vincenza Ilardi, Laura Tomassoli & Marina Barba

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  1. Wojciech Kaniewski
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  2. Vincenza Ilardi
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  4. T. Mitsky
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  5. J. Layton
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  6. Marina Barba
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Kaniewski, W., Ilardi, V., Tomassoli, L. et al. Extreme resistance to cucumber mosaic virus (CMV) in transgenic tomato expressing one or two viral coat proteins. Molecular Breeding 5, 111–119 (1999). https://doi.org/10.1023/A:1009631106873

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