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Proriatic hair follicle cells

IV Calmodulin levels in freshly isolated and cultured human scalp hair follicle cells

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Abstract

Calmodulin levels were measured by radioimmuno-assay in freshly isolated and cultured psoriatic human scalp hair follicle cells. The mean value ± SEM for calmodulin was 1.97±0.15 ng calmodulin μg-1 protein for 16 control subjects whereas calmodulin levels were significantly increased in psoriatic hair follicles, 2.93±0.26 ng calmodulin μg-1 protein (uninvolved skin) for 18 patients and 3.09±0.21 ng calmodulin μg-1 protein for involved skin derived hair follicles for 17 of these patients. In vitro, 3-week-old cultures of psoriatic keratinocytes contained less DNA and more calmodulin per DNA than their normal counterparts. When 6 week-old cultures of psoriatic and control hair follicle keratinocytes were compared, this difference disappeared. These results are related to the state of differentiation of these cultures.

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References

  1. CheungW. Y., 1980. Science 207, 19–27.

    Google Scholar 

  2. Van deKerkhofP. C. M. & VanErpP. E. J., 1983. British Journal of Dermatology 108: 217–218.

    Google Scholar 

  3. TuckerW. F. G., Mac NeilS., BleehenS. S. & TomlinsonS., 1984. The Journal of Investigative Dermatology 82: 298–299.

    Google Scholar 

  4. TuckerW. F. G., Mac NeilS., DawsonR. A., TomlinsonS. & BleehenS. S., 1985. British Journal of Dermatology 113 s. 29: 38.

    Google Scholar 

  5. FairleyJ. A., MarceloC. L., HoganV. A. & VoorheesJ. J., 1985. The Journal of Investigative Dermatology 84: 195–198.

    Google Scholar 

  6. Mac NeilS. & TuckerW. F. G., 1985. The Journal of Investigative Dermatology 85: 389.

    Google Scholar 

  7. MizumotoT., HashimotoY., HirokawaM., OhkumaN., LizukaH. & OhkawaraA., 1985. The Journal of Investigative Dermatology 85: 450–452.

    Google Scholar 

  8. ShahradP. & MarksR., 1976. British Journal of Dermatology 94: 7–12.

    Google Scholar 

  9. VermorkenA. J. M., LenoirM. C., ShrootB., KimenaiP. M. & Vermeesch MarkslagA. M. G., 1984. Molecular Biology Reports 10: 41–47.

    Google Scholar 

  10. VermorkenA. J. M., VerhagenH., GoosC. M. A. A., LenoirM. C. & ShrootB., 1985. Differentiation 29: 182–188.

    Google Scholar 

  11. Lenoir, M. C., Vermeesch Markslag, A. M. G., Goos, C. M. A. A., Shroot, B. & Vermorken, A. J. M., submitted.

  12. WeteringsP. J. J. M., VermorkenA. J. M. & BloemendalH., 1981. British Journal of Dermatology 104: 1–5.

    Google Scholar 

  13. BradfordM., 1976. Analytical Biochemistry 72: 248–254.

    Google Scholar 

  14. HukkelhovenM. W. A. C., VromansE., MarkslagA. M. G. & VermorkenA. J. M., 1981. Anticancer Research 1: 341–344.

    Google Scholar 

  15. VermorkenA. J. M., VerhagenH., Vermeesch-MarkslagA. M. G., WirtzP., BernardB. A., AsselineauD., LenoirM. C., KimenaiP. M. & ShrootB., 1985. Molecular Biology Reports 10: 205–213.

    Google Scholar 

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Authors and Affiliations

  1. Department of Biochemistry, Centre International de Recherches Dermatologiques (C.I.R.D.), Valbonne, France

    M. C. Lenoir, B. Shroot & A. J. M. Vermorken

  2. Research Unit for Cellular Differentiation and Transformation, University of Nijmegen, Nijmegen, The Netherlands

    E. Vromans & A. J. M. Vermorken

Authors
  1. M. C. Lenoir
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  2. E. Vromans
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  3. B. Shroot
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  4. A. J. M. Vermorken
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Lenoir, M.C., Vromans, E., Shroot, B. et al. Proriatic hair follicle cells. Mol Biol Rep 11, 247–252 (1986). https://doi.org/10.1007/BF00419604

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  • DOI: https://doi.org/10.1007/BF00419604

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