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Topographical analysis of yeast ribosomal DNA by cleavage with restriction endonucleases

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Abstract

Yeast ribosomal DNA (rDNA) was digested with the restriction enzymes Hind III, Hind II and a mixture of Hind II and Hind III. The cleavage products were analyzed by electrophoresis on 1.5% agarose gels. Several distinct bands could be observed, which are derived from the redundant ribosomal transcription units. They are superimposed on a rather broad smear of background DNA, representing the heterogenous ‘spacer’ sequences. From the restriction maps, together with data obtained by partial digestion, a physical map for the ribosomal transcription unit in yeast could be constructed.

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Authors and Affiliations

  1. Biochemisch Laboratorium, Vrije Universiteit, De Boelelaan 1085, Amsterdam-Buitenveldert, The Netherlands

    J. H. Meyerink, J. Retèl, R. J. Planta & F. Heidekamp

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  1. J. H. Meyerink
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  2. J. Retèl
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  3. R. J. Planta
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  4. F. Heidekamp
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Meyerink, J.H., Retèl, J., Planta, R.J. et al. Topographical analysis of yeast ribosomal DNA by cleavage with restriction endonucleases. Molecular Biology Reports 2, 393–400 (1976). https://doi.org/10.1007/BF00366261

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  • DOI: https://doi.org/10.1007/BF00366261

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