Abstract
Antifungal activity of several antimycotics has been evaluated using an automatic analyzing system (AAS), which is composed of a specially designed reaction vessel, microscopic observation system, image analyzing system, and computer program for automatic tracing of hypha growth. The agar plate was prepared on the ceiling of the reaction vessel, and spore mass of a fungus (Aspergillus niger) was inoculated onto it. After the preincubation at 28 °C for 24 h the reaction vessel was set on a microscope stage and connected to the liquid flow system. An appropriate hypha was selected for the measurement of growth process during the following steps: first contact with saline for 30 min for the adaptation, the second contact with same saline for 30 min, contact with saline containing an antimycotic substance for 60 min, and contact with flushing saline for 60 min. During a sequence of these steps, the apical tip of a growing hypha displayed on a TV monitor was followed automatically. The dynamic response of hypha to an agent was analyzed by several parameters. Morphological changes of the hypha caused by respective agents were recorded on VTR for further analysis. By using this system, the antifungal activity of antimycotics could be quantitatively determined within several hours.
Similar content being viewed by others
References
Fromtling RA. Overview of medically important antifungal azole derivatives. Clinical Microbiology Reviews 1988; 1: 187–217.
Medoff G, Brajtburg J, Kobayashi GS. Antifungal agents useful in therapy of systemic fungal infections. Ann Rev Pharmacol Toxicol 1983; 23: 303–30.
Ryley JF, Wilson RG, Gravestock MB, Poyser JP. Experimental approaches to antifungal chemotherapy. Adv Pharmco Chemo 1981; 18: 50–176.
Matsuoka H, Ii Y, Takekawa Y, Teraoka T. Evaluation of antifungal volatile compounds on the basis of the elongation rate of a single hypha. Appl Environ Microbiol 1990; 56: 3779–84.
Yamada S, Cao J, Sumita O, Kurasawa K, Kurata H, Oh K, Matsuoka H. Automatic antifungal activity analyzing system on the basis of dynamic growth process of a single hypha. Mycopathologia 1992; 118: 65–69.
Brajtburg J, Medoff G, Kobayashi GS, Elberg S. Permeabilizing and hemolytic action of large and small polyene antibiotics on human erythrocytes. Antimicrob Agents Chemother 1980; 18: 586–92.
Chen WC, Chou DL, Feingold DS. Dissociation between ion permeability and the lethal action of polyene antibiotics on Candida albicans. Antimicrob Agents Chemother 1978; 13: 914–7.
Sud IJ, Feingold DS. Heterogeneity of action mechanisms among antimycotic imidazoles. Antimicrob Agents Chemother 1981; 20: 71–4.
Van Den Bossche H, Willemsens G, Cools W, Cornelissen F, Lauwers WF, Van Cutsem JM. In vitro and in vivo effects of the antimycotic drug ketoconazole on sterol synthesis. Antimicrob Agents Chemother 1980; 17: 922–8.
Uno J, Shigematsu ML, Arai T. Primary site of action of ketoconazole on Candida albicans. Antimicrob Agents Chemother 1982; 21: 912–8.
Shigematsu ML, Uno J, Arai T. Effect of ketoconazole on isolated mitochondria from Candida albicans. Antimicrob Agents Chemother 1982; 21: 919–24.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Oh, K., Matsuoka, H., Sumita, O. et al. Evaluation of antifungal activity of antimycotics by automatic analyzing system. Mycopathologia 118, 71–81 (1992). https://doi.org/10.1007/BF00442534
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00442534