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Assay of propranolol in human and rat plasma by capillary gas chromatography with nitrogen selective detection

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Abstract

A gas Chromatographic method is described for the assay of propranolol in human and rat plasma. The extraction with diethylether is relatively simple compared to existing procedures and there is no need for clean-up. Following derivatisation with acetic acid anhydride the derivatives were determined by means of capillary gas chromatography, solid injection and nitrogen-phosphorous selective detection.

Calibration curves were linear in the concentration range from 5 to 100 ng/ml plasma. The recovery of propranolol from human and rat plasma was 80% and 100% respectively while the detection limit in both cases was approximately 1 ng/ml. There is no interference with endogenous plasma substances or metabolites and retention times are short (ca. 3 min). An application has been given. (Pharm. Weekblad Sci. Ed.2, 101–105).

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Author notes

  1. A. G. De Boer

    Present address: Department of Pharmaceutical Technology and Biopharmacy, Subfaculty of Pharmacy, University of Leiden, Wassenaarseweg 76, P.O. Box 9502, 2300, RA Leiden, The Netherlands

Authors and Affiliations

  1. Department of Pharmacology, Subfaculty of Pharmacy, University of Leiden, Wassenaarseweg 72, Leiden, The Netherlands

    A. G. De Boer, D. D. Breimer & J. M. Gubbens-Stibbe

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  1. A. G. De Boer
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  2. D. D. Breimer
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  3. J. M. Gubbens-Stibbe
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De Boer, A.G., Breimer, D.D. & Gubbens-Stibbe, J.M. Assay of propranolol in human and rat plasma by capillary gas chromatography with nitrogen selective detection. Pharmaceutisch Weekblad Scientific Edition 2, 1105–1109 (1980). https://doi.org/10.1007/BF02273266

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  • DOI: https://doi.org/10.1007/BF02273266

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