Abstract
Bean (Phaseolus vulgaris cv. Saxa) chloroplasts contain two tRNAPhe species, namely tRNAPhe1 and tRNAPhe2. By sequence determination, we show that tRNAPhe2 is identical to the previously sequenced tRNAPhe1 except for two undermodified nucleotides. By reversed-phase chromatography analyses, we demonstrate that the relative amounts of these two chloroplast tRNAsPhe vary during leaf development: in etiolated leaves the undermodified tRNAPhe2 only represents 15% of total chloroplast tRNAPhe, during development and greening it increases to reach 60% in 8-day-old leaves, and it then decreases to 9% in senescing leaves.
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Pfitzinger, H., Maréchal-Drouard, L., Pillay, D.T.N. et al. Variations during leaf development of the relative amounts of two bean (Phaseolus vulgaris) chloroplast tRNAsPhe which differ in their minor nucleotide content. Plant Mol Biol 14, 969–975 (1990). https://doi.org/10.1007/BF00019393
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DOI: https://doi.org/10.1007/BF00019393